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. 2014 Sep-Dec;18(3):379-85.
doi: 10.4103/0973-029X.151319.

Epithelial and stromal patterns of pleomorphic adenoma of minor salivary glands: A histopathological and histochemical study

Yasmin Satpathy  1 , Anita E Spadigam  1 , Anita Dhupar  1 , Shaheen Syed  1
Affiliations

Epithelial and stromal patterns of pleomorphic adenoma of minor salivary glands: A histopathological and histochemical study

Yasmin Satpathy et al. J Oral Maxillofac Pathol. 2014 Sep-Dec.

Abstract

Background: Pleomorphic adenoma (PA) accounts for 45-74% of all the salivary gland neoplasms, of which 40-70% are present in minor salivary glands. Studies have depicted variations in histological typing and classification of these tumors. Its pleomorphism is attributed to the cytological differentiations of the epithelial components and the diverse stromal components. Biochemical investigations of saliva have revealed "mucins" to be its main component. Mucins reflect in their composition, the functional state of the mucosa, both in health and disease. Many reviews on histochemical classification and identification have been put forward to explain the intricacies of mucins; however, no attempts have been made to classify salivary gland tumors based on their mucin profiles and assess its prognostic significance. Thus, this study was executed to analyze the clinical, histopathological and histochemical behavior of PA of minor salivary glands and decipher a correlation.

Materials and methods: Twenty-six diagnosed cases of PA of minor salivary glands and five controls of normal minor salivary glands of the hard palate were included in the study. Blocks were retrieved, sectioned and stained with hematoxylin and eosin (H and E) stain as well as combined Alcian blue (AB)-periodic acid-Schiff (PAS) stains.

Results: The stained slides revealed an array of epithelial and stromal patterns and varying heterogeneity of mucin expression of normal and neoplastic minor salivary glands.

Conclusion: The study elucidated the role of mucins in tumorigenesis and its prognostic implications.

Keywords: Alcian blue-PAS stain; Mucins; Pleomorphic adenoma.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Photomicrograph showing tumoral off shoots within the capsule of pleomorphic adenoma (H&E stain ×100)
Figure 2
Figure 2
Photomicrograph showing myxoid stroma in pleomorphic adenoma (H&E stain, ×100)
Figure 3
Figure 3
Photomicrograph showing osteoid-like matrix/trabecular pattern (Alcian blue-periodic acid-Schiff (AB-PAS) stain, ×100)
Figure 4
Figure 4
Photomicrograph showing cellular variant of PA (H&E stain, ×400)
Figure 5
Figure 5
Photomicrograph showing cellular variant of PA (AB-PAS stain, ×400)
Figure 6
Figure 6
Photomicrograph showing cellular variant of PA (AB-PAS stain, ×400)
Figure 7
Figure 7
Photomicrograph showing AB positive chondromyxoid areas (AB-PAS stain, ×100)
Figure 8
Figure 8
Photomicrograph showing mucous acini in myxoid tumors (AB-PAS stain, ×400)
Figure 9
Figure 9
Photomicrograph showing mucous acini in cellular tumors (AB-PAS stain, ×400)
See this image and copyright information in PMC

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References

    1. Henriksson G, Westrin KM, Carlsoo B, Silfverswärd C. Recurrent primary pleomorphic adenomas of salivary gland origin: Intrasurgical rupture, histopathologic features and pseudopodia. Cancer. 1998;82:617–20. - PubMed
    1. Hamada T, Matsukita S, Goto M, Kitajima S, Batra SK, Irimura T, et al. Mucin expression in pleomorphic adenoma of salivary gland: A potential role for MUC1 as a marker to predict recurrence. J Clin Pathol. 2004;57:813–21. - PMC - PubMed
    1. Zalewska A, Zwierz K, Zółkowski K, Gindzieński A. Structure and biosynthesis of human salivary mucins. Acta Biochim Pol. 2000;47:1067–79. - PubMed
    1. Hand AR, Pathmanathan D, Field RB. Morphological features of minor salivary gland. Arch Oral Biol. 1999;44:S3–10. - PubMed
    1. Totty BA. Mucins. In: Bancroft JD, Gamble M, editors. Theory and Practice of Histological Techniques. 5th edition. Edinburgh: Churchill Livingstone; 2002. pp. 112–116.