Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Apr 8:6:31.
doi: 10.4103/2008-7802.154773. eCollection 2015.

Association of adiponectin gene polymorphism with adiponectin levels and risk for insulin resistance syndrome

Jai Prakash  1 , Balraj Mittal  2 , Shally Awasthi  3 , Neena Srivastava  4
Affiliations

Association of adiponectin gene polymorphism with adiponectin levels and risk for insulin resistance syndrome

Jai Prakash et al. Int J Prev Med. .

Abstract

Background: Adiponectin is an abundant adipose tissue-derived protein with anti-atherogenic, anti-inflammatory and antidiabetic properties. Plasma adiponectin levels are decreased in obesity, type 2 diabetes, and coronary artery disease and low adiponectin levels also predict insulin resistance (IR).

Methods: Case-control study in which 642 male and female subjects were participated from the North Indian population. Lipid, insulin, leptin and adiponectin level were estimated using standard protocols by commercially available test kits. Single nucleotide polymorphisms +45T>G and +276G>T of the AMP1 (adiponectin) gene was genotyped by polymerase chain reaction restriction fragment length polymorphism method.

Results: Levels of adiponectin, insulin, homeostasis model assessment-IR index (HOMA-IR index), systolic blood pressure and fat mass showed significant differences between male and female subjects. Serum adiponectin level showed highly significant association with both the +45 and the +276 genotypes. The common haplotype triglyceride (TG) showed a significantly lower adiponectin value than other haplotypes (P = 0.0001). A clear trend of decreasing adiponectin levels per copy of the common haplotype was observed. Nonobese insulin sensitive subjects showed a higher adiponectin value (P = 0.0006) than nonobese insulin resistant subjects. The values of blood pressure, adiponectin, insulin, HOMA-IR, total-cholesterol, and low-density lipoprotein-cholesterol significantly associated with TG haplotype.

Conclusions: We observed the very strong association of the adiponectin 45-276 genotypes and haplotypes with adiponectin levels in healthy north Indian population and TG haplotypes also associated with metabolic parameters of the IR syndrome.

Keywords: AMP1; Adiponectin; haplotype; insulin resistance; polymorphism.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest: None declared.

References

    1. Nigro E, Scudiero O, Monaco ML, Palmieri A, Mazzarella G, Costagliola C, et al. New insight into adiponectin role in obesity and obesity-related diseases. Biomed Res Int 2014. 2014 658913. - PMC - PubMed
    1. Arikoglu H, Ozdemir H, Kaya DE, Ipekci SH, Arslan A, Kayis SA, et al. The adiponectin variants contribute to the genetic background of type 2 diabetes in Turkish population. Gene. 2014;534:10–16. - PubMed
    1. Marseglia L, Manti S, D’Angelo G, Nicotera A, Parisi E, Di Rosa G, et al. Oxidative stress in obesity: A critical component in human diseases. Int J Mol Sci. 2014;16:378–400. - PMC - PubMed
    1. Francke S, Manraj M, Lacquemant C, Lecoeur C, Leprêtre F, Passa P, et al. A genome-wide scan for coronary heart disease suggests in Indo-Mauritians a susceptibility locus on chromosome 16p13 and replicates linkage with the metabolic syndrome on 3q27. Hum Mol Genet. 2001;10:2751–65. - PubMed
    1. Xita N, Tsatsoulis A. Adiponectin in diabetes mellitus. Curr Med Chem. 2012;19:5451–8. - PubMed