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. 2015 May 7;10(5):e0126398.
doi: 10.1371/journal.pone.0126398. eCollection 2015.

Chromosomal Behavior during Meiosis in the Progeny of Triticum timopheevii × Hexaploid Wild Oat

Hongzhou An  1 , Mei Hu  1 , Pengfei Li  1 , Guangdong Geng  2 , Qingqin Zhang  1 , Suqin Zhang  1
Affiliations

Chromosomal Behavior during Meiosis in the Progeny of Triticum timopheevii × Hexaploid Wild Oat

Hongzhou An et al. PLoS One. .

Abstract

The meiotic behavior of pollen mother cells (PMCs) of the F2 and F3 progeny from Triticum timopheevii × hexaploid wild oat was investigated by cytological analysis and sequential C-banding-genomic in situ hybridization (GISH) in the present study. A cytological analysis showed that the chromosome numbers of the F2 and F3 progeny ranged from 28 to 41. A large number of univalents, lagging chromosomes, chromosome bridges and micronuclei were found at the metaphase I, anaphase I, anaphase II and tetrad stages in the F2 and F3 progeny. The averages of univalents were 3.50 and 2.73 per cell, and those of lagging chromosomes were 3.37 and 1.87 in the F2 and F3 progeny, respectively. The PMC meiotic indices of the F2 and F3 progeny were 12.22 and 20.34, respectively, indicating considerable genetic instability. A sequential C-banding-GISH analysis revealed that some chromosomes and fragments from the hexaploid wild oat were detected at metaphase I and anaphase I in the progeny, showing that the progeny were of true intergeneric hybrid origin. The alien chromosomes 6A, 7A, 3C and 2D were lost during transmission from F2 to F3. In addition, partial T. timopheevii chromosomes appeared in the form of univalents or lagging chromosomes, which might result from large genome differences between the parents, and the wild oat chromosome introgression interfered with the wheat homologues' normally pairing.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. The percentage of irregular chromosomes at metaphase I, anaphase I and telophase I in the pollen mother cells’ (PMCs) in the Triticum timopheevii × hexaploid wild oat F2 and F3 generations, respectively.
A, The x-axis shows the numbers of univalents in each PMC at metaphase I. B, The x-axis shows the numbers of lagging chromosomes and chromosome bridges in each PMC at anaphase I. C, The x-axis shows the numbers of micronuclei in each PMC at telophase I.
Fig 2
Fig 2. The pollen mother cells’ (PMCs) meiotic behavior in some Triticum timopheevii × hexaploid wild oat F2 plants.
A and B, Anaphase I in the plant TPF2-1. Arrows show lagging chromosomes (A) and chromosome bridge (B). C, Anaphase I in the plant TPF2-2. Arrows show lagging chromosomes. D, Telophase I in the plant TPF2-1. Arrows show lagging univalent. E, Anaphase II in the plant TPF2-2, showing daughter cells from the first division were non-synchronous. Scale bar = 10 μm.
Fig 3
Fig 3. The percentage of pollen mother cells’ (PMCs) with micronuclei in the Triticum timopheevii × hexaploid wild oat F2 and F3 generations at telophase II.
Fig 4
Fig 4. C-banding pattern of root tip cells in T. timopheevii (A) and the hexaploid Avena fatua L. (B).
Scale bar = 10 μm.
Fig 5
Fig 5. Sequential C-banding- genomic in situ hybridization (GISH) analysis of the pollen mother cells’ (PMCs) of the Triticum timopheevii × hexaploid wild oat F2 and F3 progeny.
A and B, Sequential C-banding-GISH analysis of the PMCs in an F2 plant at metaphase I. C and D, Sequential C-banding-GISH analysis of the PMCs in an F3 plant at metaphase I. E and F, Sequential C-banding-GISH analysis of the PMCs in an F2 plant at anaphase I. G and H, Sequential C-banding-GISH analysis of the PMCs in an F3 plant at anaphase I. Arrows show the chromosomes from the hexaploid A. fatua L.: red, black and green arrows show A-, C- and D- genome chromosomes, respectively. The chromosomes were counterstained with DAPI (blue), and hybridization sites are in red. Scale bar = 10 μm.
See this image and copyright information in PMC

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