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Review
. 2015:557:329-48.
doi: 10.1016/bs.mie.2014.12.005. Epub 2015 Mar 17.

Solution NMR Structure Determination of Polytopic α-Helical Membrane Proteins: A Guide to Spin Label Paramagnetic Relaxation Enhancement Restraints

Linda Columbus  1 , Brett Kroncke  2
Affiliations
Review

Solution NMR Structure Determination of Polytopic α-Helical Membrane Proteins: A Guide to Spin Label Paramagnetic Relaxation Enhancement Restraints

Linda Columbus et al. Methods Enzymol. 2015.

Abstract

Solution nuclear magnetic resonance structures of polytopic α-helical membrane proteins require additional restraints beyond the traditional Nuclear Overhauser Effect (NOE) restraints. Several methods have been developed and this review focuses on paramagnetic relaxation enhancement (PRE). Important aspects of spin labeling, PRE measurements, structure calculations, and structural quality are discussed.

Keywords: Membrane proteins; Nitroxide spin label; Nuclear magnetic resonance; Paramagnetic relaxation enhancement; Polytopic; Site-directed spin labeling; α-Helical.

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