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. 1989 Jul-Sep;19(3):259-66.
doi: 10.1007/BF02871815.

One-step purification by high-performance liquid chromatography of retinal S-antigen

C Molinotti  1 , P Puddu, S Perugini, P Barboni, R Biagi
Affiliations

One-step purification by high-performance liquid chromatography of retinal S-antigen

C Molinotti et al. Ric Clin Lab. 1989 Jul-Sep.

Abstract

A number of subsequent chromatographic techniques are commonly used, over a period of a few days, in order to obtain pure retinal S-antigen (S-Ag), a 50,000-dalton molecular weight protein with strong uveitopathogenic properties. A rapid high-performance liquid chromatographic technique employing a TSK-G3000 SWG gel-filtration column is described for the purification of bovine S-Ag. This single chromatographic step produced S-Ag preparations of high purity and recovery when compared to the conventionally isolated S-Ag.

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