Cobalamin and its binding protein in rat milk
- PMID: 2595247
- DOI: 10.3109/00365518909089132
Cobalamin and its binding protein in rat milk
Abstract
Cobalamin and its binding protein, haptocorrin, are present in rat milk throughout the lactation period. The concentration of cobalamin is approximately 0.3-times the concentration of the unsaturated binding protein. The concentration of the unsaturated cobalamin-binding protein varies between 18 nmol l-1 and 16 nmol l-1. The binding protein has a Stokes radius of 2.49 nm when saturated with cobalamin and 2.61 nm when unsaturated. It binds cobalamin over a broad range of pH and is able to bind cobinamide also. With immunohistochemistry, we find haptocorrin immunoreactivity in the mammary glands and in the sebaceous glands of the nipple.
Similar articles
-
Characterization and immunohistochemical localization of rat salivary cobalamin-binding protein and comparison with human salivary haptocorrin.Biochim Biophys Acta. 1985 Feb 15;838(2):264-9. doi: 10.1016/0304-4165(85)90088-1. Biochim Biophys Acta. 1985. PMID: 3970971
-
Cobalamin and haptocorrin in human milk and cobalamin-related variables in mother and child: a 9-mo longitudinal study.Am J Clin Nutr. 2013 Aug;98(2):389-95. doi: 10.3945/ajcn.113.058479. Am J Clin Nutr. 2013. PMID: 23783295
-
Transcobalamin from cow milk: isolation and physico-chemical properties.Biochim Biophys Acta. 1996 Jan 4;1292(1):113-9. doi: 10.1016/0167-4838(95)00173-5. Biochim Biophys Acta. 1996. PMID: 8547333
-
Vitamin B12 metabolism and status during pregnancy, lactation and infancy.Adv Exp Med Biol. 1994;352:173-86. doi: 10.1007/978-1-4899-2575-6_14. Adv Exp Med Biol. 1994. PMID: 7832046 Review.
-
Cobalamin metabolism and its clinical aspects.Clin Sci (Lond). 1984 Feb;66(2):113-21. doi: 10.1042/cs0660113. Clin Sci (Lond). 1984. PMID: 6420106 Review. No abstract available.
Cited by
-
Rat transcobalamin: cloning and regulation of mRNA expression.J Physiol. 2004 Apr 15;556(Pt 2):623-35. doi: 10.1113/jphysiol.2003.058727. Epub 2004 Jan 14. J Physiol. 2004. PMID: 14724191 Free PMC article.
-
Processing of alkylcobalamins in mammalian cells: A role for the MMACHC (cblC) gene product.Mol Genet Metab. 2009 Aug;97(4):260-6. doi: 10.1016/j.ymgme.2009.04.005. Epub 2009 Apr 16. Mol Genet Metab. 2009. PMID: 19447654 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources