A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila

Jiang Xu¹, Xingjie Ren², Jin Sun², Xia Wang², Huan-Huan Qiao², Bo-Wen Xu², Lu-Ping Liu³, Jian-Quan Ni⁴

Affiliations

¹ Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China; Tsinghua Fly Center, Tsinghua University, Beijing 100084, China; School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China; College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
² Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
³ Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China; Tsinghua Fly Center, Tsinghua University, Beijing 100084, China.
⁴ Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China. Electronic address: nijq@mail.tsinghua.edu.cn.

PMID: 25953352
DOI: 10.1016/j.jgg.2015.02.007

Review

A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila

Jiang Xu et al. J Genet Genomics. 2015.

. 2015 Apr 20;42(4):141-9.

doi: 10.1016/j.jgg.2015.02.007. Epub 2015 Mar 12.

Authors

Jiang Xu¹, Xingjie Ren², Jin Sun², Xia Wang², Huan-Huan Qiao², Bo-Wen Xu², Lu-Ping Liu³, Jian-Quan Ni⁴

Affiliations

¹ Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China; Tsinghua Fly Center, Tsinghua University, Beijing 100084, China; School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China; College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
² Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
³ Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China; Tsinghua Fly Center, Tsinghua University, Beijing 100084, China.
⁴ Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China. Electronic address: nijq@mail.tsinghua.edu.cn.

PMID: 25953352
DOI: 10.1016/j.jgg.2015.02.007

Abstract

The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.

Keywords: CRISPR; Cas9; Drosophila; Genome editing; Off-target; sgRNA.

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A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila

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A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila

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