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Review
. 2015 Apr 20;42(4):141-9.
doi: 10.1016/j.jgg.2015.02.007. Epub 2015 Mar 12.

A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila

Jiang Xu  1 , Xingjie Ren  2 , Jin Sun  2 , Xia Wang  2 , Huan-Huan Qiao  2 , Bo-Wen Xu  2 , Lu-Ping Liu  3 , Jian-Quan Ni  4
Affiliations
Review

A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila

Jiang Xu et al. J Genet Genomics. .

Abstract

The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.

Keywords: CRISPR; Cas9; Drosophila; Genome editing; Off-target; sgRNA.

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