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. 2015 Mar;9(3):ZM01-3.
doi: 10.7860/JCDR/2015/11083.5615. Epub 2015 Mar 1.

Transit fixatives: an innovative study

Rajanikanth M  1 , Ravi Prakash A  2 , Sreenath G  3 , Sonia Bai Jk  4 , Shyam Ndvn  5
Affiliations

Transit fixatives: an innovative study

Rajanikanth M et al. J Clin Diagn Res. 2015 Mar.

Abstract

Background: Universally accepted fixative is 10% formalin which has been used for preserving the tissues and their architecture. In certain conditions, formalin might not be readily available for immediate fixation. We here by explore more economical, eco-friendly and easily available solutions that can be used as transit media/ transporting media for tissue specimens.

Materials and methods: The study included commonly available solutions like Spirit, Saline, Betadine solution, Hydrogen peroxide (H2O2), Local anesthesia (L.A), Rose water, Coconut oil, Coconut water, Ice cold water, Honey and Milk while keeping formalin as control. The fresh tissue sample was cut into multiple bits and placed in different containers for a period of 8 hours before transferring to formalin solution.

Conclusion: Transit fixatives are very important in certain situations where formalin is not readily available. These fixatives can be used to fix the tissues for a period of at least 8 hours without causing any damage or distortion before they are fixed in formalin solution.

Keywords: Biopsy; Formalin; Transporting media.

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Figures

[Table/Fig-2]:
[Table/Fig-2]:
Hematoxylin & Eosin section of tissue fixed in Formalin
[Table/Fig-3]:
[Table/Fig-3]:
Hematoxylin & Eosin section of tissue fixed in HONEY prior to formalin fixation
[Table/Fig-4]:
[Table/Fig-4]:
Hematoxylin & Eosin section of tissue fixed in LOCAL ANESTHESIA prior to formalin
[Table/Fig-5]:
[Table/Fig-5]:
Hematoxylin & Eosin section of tissue fixed in H2O2 prior to formalin
[Table/Fig-6]:
[Table/Fig-6]:
Hematoxylin & Eosin section of tissue fixed in COCONUT OIL prior to formalin
See this image and copyright information in PMC

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