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. 2015 Jul:114:51-3.
doi: 10.1016/j.mimet.2015.05.005. Epub 2015 May 6.

TM7 detection in human microbiome: Are PCR primers and FISH probes specific enough?

Maria V Sizova  1 , Sebastian N Doerfert  1 , Ekaterina Gavrish  1 , Slava S Epstein  2
Affiliations

TM7 detection in human microbiome: Are PCR primers and FISH probes specific enough?

Maria V Sizova et al. J Microbiol Methods. 2015 Jul.

Abstract

TM7 appears important and omnipresent because it is repeatedly detected by molecular techniques in diverse environments. Here we report that most of primers and FISH probes thought to be TM7-specific do hybridize with multiple species from oral and vaginal cavity. This calls for re-examination of TM7 distribution and abundance.

Keywords: Microbial cultivation; Oral microbiome; TM7.

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Conflict of interest statement

Conflict of interest

The authors declare no conflict of interest.

Figures

Fig 1
Fig 1
The UV microscopic images of DTAF stained filamentous and rod shaped anaerobic bacteria that showed false positive results with published TM7-“specific” 16S rDNA primers and FISH probes.
Fig 1
Fig 1
The UV microscopic images of DTAF stained filamentous and rod shaped anaerobic bacteria that showed false positive results with published TM7-“specific” 16S rDNA primers and FISH probes.
See this image and copyright information in PMC

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