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Comment
. 2015 May 7;16(5):453-4.
doi: 10.1016/j.stem.2015.04.018.

Forces of Change: Mechanics Underlying Formation of Functional 3D Organ Buds

Paul J Wrighton  1 , Laura L Kiessling  2
Affiliations
Comment

Forces of Change: Mechanics Underlying Formation of Functional 3D Organ Buds

Paul J Wrighton et al. Cell Stem Cell. .

Abstract

3D organ buds that can recapitulate organ function have myriad applications for regenerative and personalized medicine. Here, Takebe et al. (2015) describe a generalized method for organ bud formation, demonstrating that mechanosensitive mesenchymal stem cells drive condensation of heterotypic cell mixtures to create buds from diverse organs.

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Figures

Figure 1
Figure 1. Mechanical Properties of the Substratum Drive Contraction of MSCs to Form Heterotypic Cell Condensates
HPSC-derived hepatic endoderm (or one or more functional cell type) is combined with MSCs and HUVECs, and the mixture is cultured in 2D on hydrogel substrata of varying stiffness. The MSCs respond to the mechanical properties of the substrate and condensation occurs on the surface with “Just Right” stiffness, forming a liver bud (or bud corresponding to the functional cell type).
See this image and copyright information in PMC

Comment on

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