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. 2015 Jun 26:411:33-6.
doi: 10.1016/j.carres.2015.04.004. Epub 2015 Apr 17.

Structure and gene cluster of the O-antigen of Escherichia coli O140

Andrei V Perepelov  1 , Quan Wang  2 , Sof'ya N Senchenkova  3 , Zhu Mei  2 , Alexander S Shashkov  3 , Lei Wang  2 , Yuriy A Knirel  3
Affiliations

Structure and gene cluster of the O-antigen of Escherichia coli O140

Andrei V Perepelov et al. Carbohydr Res. .

Abstract

An acidic O-polysaccharide (O-antigen) was isolated from the lipopolysaccharide of Escherichia coli O140 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the branched hexasaccharide repeating unit was established: [Formula: see text]. The O-antigen gene cluster of E. coli O140 was sequenced. The gene functions were tentatively assigned by a comparison with sequences in the available databases and found to be in full agreement with the E. coli O140 polysaccharide structure.

Keywords: Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-antigen; O-antigen gene cluster.

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