Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Aug:115:54-6.
doi: 10.1016/j.mimet.2015.05.010. Epub 2015 May 9.

Assessment of four protocols for rapid bacterial identification from positive blood culture pellets by matrix-assisted laser desorption ionization-time of flight mass spectrometry (Vitek® MS)

Jean Thomin  1 , Guillaume Ghislain Aubin  2 , Fabrice Foubert  3 , Stéphane Corvec  4
Affiliations

Assessment of four protocols for rapid bacterial identification from positive blood culture pellets by matrix-assisted laser desorption ionization-time of flight mass spectrometry (Vitek® MS)

Jean Thomin et al. J Microbiol Methods. 2015 Aug.

Abstract

In this study, we developed and compared four protocols to prepare a bacterial pellet from 944 positive blood cultures for direct MALDI-TOF mass spectrometry Vitek® MS analysis. Protocol 4, tested on 200 monomicrobial samples, allowed 83% of bacterial identification. This easy, fast, cheap and accurate method is promising in daily practice, especially to limit broad range antibiotic treatment.

Keywords: Blood bottle culture; MALDI-TOF; Rapid identification.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources