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. 1989 Oct 15;263(2):365-70.
doi: 10.1042/bj2630365.

Human serum amyloid A protein. Behaviour in aqueous and urea-containing solutions and antibody production

A F Strachan  1 , E G Shephard, D U Bellstedt, G A Coetzee, D R van der Westhuyzen, F C de Beer
Affiliations

Human serum amyloid A protein. Behaviour in aqueous and urea-containing solutions and antibody production

A F Strachan et al. Biochem J. .

Abstract

Human serum amyloid A protein (apo-SAA) can be prepared by gel filtration of delipidated acute-phase high-density lipoprotein in the presence of urea. The resultant apo-SAA is soluble (greater than 90% solubility) in a wide range of buffer solutions, with all of the six major isoforms of apo-SAA being equally soluble. In urea-containing solutions the isoforms behave qualitatively differently in various urea concentrations, probably reflecting subtle primary-structure variations. The higher-pI isoforms are only completely unfolded at greater than 7 M-urea. By immunizing with apo-SAA adsorbed to acid-treated bacteria (Salmonella minnesota R595), high-titre antibodies can easily be elicited in rabbits.

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References

    1. J Exp Med. 1986 Mar 1;163(3):499-510 - PubMed
    1. J Immunol Methods. 1985 Nov 7;83(2):217-25 - PubMed
    1. J Immunol Methods. 1987 Apr 16;98(2):249-55 - PubMed
    1. Scand J Clin Lab Invest. 1987 Oct;47(6):619-26 - PubMed
    1. Biochem J. 1988 Feb 15;250(1):203-7 - PubMed

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