Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Nov 1;263(3):855-9.
doi: 10.1042/bj2630855.

Kinetic analysis of protein modification reactions at equilibrium

E T Rakitzis  1
Affiliations

Kinetic analysis of protein modification reactions at equilibrium

E T Rakitzis. Biochem J. .

Abstract

A kinetic analysis is presented of reactions of protein modification, and/or of modification-induced enzyme inactivation, which can formally be described by a single exponential function, or by a summation of two exponential functions, of reaction time plus a constant term. The reaction schemes compatible with the kinetic formalism of these cases are given, and a simple kinetic criterion is described whereby the identification of one of these cases, strong negative protein modification co-operativity, may be carried out. The treatment outlined in this paper is applied to a case from the literature, the inactivation of glyceraldehyde-3-phosphate dehydrogenase by butane-2,3-dione [Asriyants, Benkevich & Nagradova (1983) Biokhimiya (Engl. Transl.) 48, 164-171].

PubMed Disclaimer

References

    1. Eur J Biochem. 1979 Jan 15;93(2):375-83 - PubMed
    1. J Biol Chem. 1965 Jul;240:2921-34 - PubMed
    1. Biochemistry. 1971 Nov 23;10(24):4538-44 - PubMed
    1. Biochemistry. 1971 Nov 23;10(24):4544-52 - PubMed
    1. J Mol Biol. 1973 Oct 15;80(1):41-62 - PubMed