Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2015 Jul 2;34(13):1740-2.
doi: 10.15252/embj.201591894. Epub 2015 May 13.

Simply the right time to turn on insulin

Francesca M Spagnoli  1
Affiliations
Comment

Simply the right time to turn on insulin

Francesca M Spagnoli. EMBO J. .

Abstract

Recent research has made important progress in the directed differentiation of human pluripotent stem cells into insulin-producing beta-like cells in vitro. A new study published in this issue of The EMBO Journal reports that timely induction of NEUROG3 expression in pancreatic progenitors is a crucial checkpoint for generation of functional human beta cells.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Directed differentiation of hES cells into mature beta-like cells Simplified diagram describing the new strategy reported by Russ et al (2015) for in vitro differentiation of hESCs into pancreatic beta-like cells. The addition of BMP inhibitors at pancreatic progenitor stage (PP) ensures precise temporal activation of NEUROG3 and favors generation of monohormonal insulin-secreting cells versus polyhormonal cells. Commonly used differentiation strategy is boxed in light gray, wherein earlier inhibition of BMP signaling leads to premature endocrine commitment and higher number of polyhormonal cells.
See this image and copyright information in PMC

Comment on

References

    1. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2013;36(Suppl. 1):S67–S74. - PMC - PubMed
    1. Chung WS, Andersson O, Row R, Kimelman D, Stainier DY. Suppression of Alk8-mediated Bmp signaling cell-autonomously induces pancreatic beta-cells in zebrafish. Proc Natl Acad Sci USA. 2010;107:1142–1147. - PMC - PubMed
    1. D'Amour K, Bang A, Eliazer S, Kelly O, Agulnick A, Smart N, Moorman M, Kroon E, Carpenter M, Baetge E. Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol. 2006;24:1392–1401. - PubMed
    1. Johansson K, Dursun U, Jordan N, Gu G, Beermann F, Gradwohl G, Grapin-Botton A. Temporal control of neurogenin3 activity in pancreas progenitors reveals competence windows for the generation of different endocrine cell types. Dev Cell. 2007;12:457–465. - PubMed
    1. Nostro M, Sarangi F, Yang C, Holland A, Elefanty A, Stanley E, Greiner D, Keller G. Efficient Generation of NKX6-1(+) Pancreatic Progenitors from Multiple Human Pluripotent Stem Cell Lines. Stem Cell Reports. 2015;4:591–604. - PMC - PubMed

LinkOut - more resources