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. 2015 Feb 1;8(2):1737-42.
eCollection 2015.

Anti-tumor effect and mechanism of cyclooxygenase-2 inhibitor through matrix metalloproteinase 14 pathway in PANC-1 cells

Siyuan Li  1 , Zhuoyu Gu  1 , Zhiwei Xiao  1 , Ting Zhou  2 , Jun Li  3 , Kan Sun  3
Affiliations

Anti-tumor effect and mechanism of cyclooxygenase-2 inhibitor through matrix metalloproteinase 14 pathway in PANC-1 cells

Siyuan Li et al. Int J Clin Exp Pathol. .

Abstract

Objective: To investigate whether celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, can attenuate proliferation, migration, invasion and MMP-14 expression in pancreatic cancer cells PANC-1 and the possible anti-tumor mechanism of celecoxib.

Methods: Human pancreatic cancer cell line PANC-1 cells were treated with diverse concentrations of celecoxib (20, 60, 100 μmol/L). Cell proliferation, invasion and migration capabilities were measured by MTT colorimetry, transwell invasion assay, and scratch assay separately. At the same time, the protein expression of COX-2 and MMP-14 was assessed by ELISA.

Results: The capabilities of proliferation, invasion and migration in PANC-1 cells were attenuated in a concentration-dependent manner after treated with celecoxib, followed by the down-regulation of the protein expression of COX-2 and MMP-14. In addition, MMP-14 expression was significantly positively correlated with COX-2 expression.

Conclusions: COX-2 inhibitor celecoxib can inhibit the proliferation, invasion and migration of PANC-1 cells via down-regulating the expression of MMP-14 in a concentration-dependent manner, thus contributing to its anti-tumor effect in pancreatic cancer.

Keywords: Pancreatic cancer; celecoxib; cyclooxygenase-2; matrix metalloproteinase-14.

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Figures

Figure 1
Figure 1
Effects of Celebrex on proliferation ability of PANC-1 cell.
Figure 2
Figure 2
Effects of Celebrex on invasive and migration ability of PANC-1 cell. A, E: Solvent control; B, F: Celecoxib 20 μmol/L; C, G: Celecoxib 60 μmol/L; D, H: Celecoxib 100 μmol/L.
Figure 3
Figure 3
Morphology of PANC-1 cell. A: solvent control; B: 60 μmol/L celecoxib group; C: 100 μmol/L celecoxib group.
Figure 4
Figure 4
The standard curve and protein expression levels of COX-2 and MMP-14. *P<0.05.
See this image and copyright information in PMC

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