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. 2015 Feb 1;8(2):1973-8.
eCollection 2015.

Galectin-1 reduction and changes in T regulatory cells may play crucial roles in patients with unexplained recurrent spontaneous abortion

Minjun Wu  1 , Ping Liu  1 , Licun Cheng  1
Affiliations

Galectin-1 reduction and changes in T regulatory cells may play crucial roles in patients with unexplained recurrent spontaneous abortion

Minjun Wu et al. Int J Clin Exp Pathol. .

Abstract

To investigate the changes of Galectin-1 and T-lymphocyte phenotypes in unexplained recurrent spontaneous abortion (URSA). Totally 60 participants were recruited and divided into 3 groups in average: pregnant patients with URSA (URSA group), normal early pregnant women with induced abortion (IA group) and normal non-pregnant women (control group). After the tissue and blood sample were collected, Galectin-1 was measured using enzyme-linked immunosorbent assay. Then the proportion of T regulatory cells was determined by flow cytometry. The expression levels of Galectin-1 in IA group and URSA group was significantly higher than that in the control group (24.30 ± 3.06 and 6.23 ± 2.41 vs. 1.30 ± 0.66, P < 0.05). Besides, the expression level of Galectin-1 in URSA group was lower than that in IA group (P < 0.05). The percentage of CD4(+)CD25(+)Foxp3(+) Tregs was lower in URSA group than IA group (0.77 ± 0.31 vs. 1.00 ± 0.35, P < 0.05) and the ratio of CD4(+)CD25(+)Foxp3(+)/CD4(+) in URSA group was also obviously lower than that in IA and control group (P < 0.05). Galectin-1 and CD4(+)CD25(+)Foxp3(+) may play essential roles in maintaining a normal pregnancy and their reduction may involve in the pathogenesis of URSA.

Keywords: CD4+CD25+Foxp3+; T cells; decidual tissue.

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Figures

Figure 1
Figure 1
Galectin-1 expression in participants detected by Galectin-1 ELISA commercial kit. *P < 0.05 vs. control group; #P < 0.05 vs. IA group.
Figure 2
Figure 2
T-lymphocyte subpopulations detected by flow cytometry. (A-C) were the percentage of CD4+, CD4+CD25+, or CD4+CD25+Foxp3+ Tregs in peripheral blood. (D) was the ratio of CD4+CD25+Foxp3+/CD4+ Tregs. *P < 0.05 vs. control group; #P < 0.05 vs. IA group.
See this image and copyright information in PMC

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