doi: 10.1016/0304-4165(89)90160-8.
Fluorescence lifetime distributions of DNA-4',6-diamidino-2-phenylindole complex
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- PMID: 2597690
- DOI: 10.1016/0304-4165(89)90160-8
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Fluorescence lifetime distributions of DNA-4',6-diamidino-2-phenylindole complex
Biochim Biophys Acta.
.
Free article
Abstract
Time-resolved fluorescence of 4',6-diamidino-2-phenylindole (DAPI) complexes show that for a homogeneous polymer (polyd(AT) or polyd(A).polyd(T)) at high P/D (phosphate/dye) ratio, a single exponential component adequately describes the fluorescence decay. For the AT polymers at low P/D ratio or for native DNA, the decay cannot be described by a single-exponential term. A continuous distribution of lifetime values of Gaussian shape gives a good fit to the decay data. We propose that the lifetime distribution method for the analysis of the fluorescence decay of DNA-DAPI complexes provides a useful method of characterizing the microheterogeneity of site binding.
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