Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Dec 8;993(2-3):174-8.
doi: 10.1016/0304-4165(89)90160-8.

Fluorescence lifetime distributions of DNA-4',6-diamidino-2-phenylindole complex

M L Barcellona  1 , E Gratton
Affiliations
Free article

Fluorescence lifetime distributions of DNA-4',6-diamidino-2-phenylindole complex

M L Barcellona et al. Biochim Biophys Acta. .
Free article

Abstract

Time-resolved fluorescence of 4',6-diamidino-2-phenylindole (DAPI) complexes show that for a homogeneous polymer (polyd(AT) or polyd(A).polyd(T)) at high P/D (phosphate/dye) ratio, a single exponential component adequately describes the fluorescence decay. For the AT polymers at low P/D ratio or for native DNA, the decay cannot be described by a single-exponential term. A continuous distribution of lifetime values of Gaussian shape gives a good fit to the decay data. We propose that the lifetime distribution method for the analysis of the fluorescence decay of DNA-DAPI complexes provides a useful method of characterizing the microheterogeneity of site binding.

PubMed Disclaimer