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. 2015 Jul;12(7):645-8.
doi: 10.1038/nmeth.3405. Epub 2015 May 18.

In vivo single-cell labeling by confined primed conversion

William P Dempsey  1 , Lada Georgieva  1 , Patrick M Helbling  1 , Ali Y Sonay  1 , Thai V Truong  2 , Michel Haffner  1 , Periklis Pantazis  1
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In vivo single-cell labeling by confined primed conversion

William P Dempsey et al. Nat Methods. 2015 Jul.

Abstract

Spatially confined green-to-red photoconversion of fluorescent proteins with high-power, pulsed laser illumination is negligible, thus precluding optical selection of single cells in vivo. We report primed conversion, in which low-power, dual-wavelength, continuous-wave illumination results in pronounced photoconversion. With a straightforward addition to a conventional confocal microscope, we show confined primed conversion in living zebrafish and reveal the complex anatomy of individual neurons packed between neighboring cells.

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