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. 2015 May 18;16(5):11385-97.
doi: 10.3390/ijms160511385.

Polymorphisms of the Ovine BMPR-IB, BMP-15 and FSHR and Their Associations with Litter Size in Two Chinese Indigenous Sheep Breeds

Weimin Wang  1 , Shijia Liu  2 , Fadi Li  3 , Xiangyu Pan  4 , Chong Li  5 , Xiaoxue Zhang  6 , Youji Ma  7   8 , Yongfu La  9 , Rui Xi  10 , Tingfu Li  8   11
Affiliations

Polymorphisms of the Ovine BMPR-IB, BMP-15 and FSHR and Their Associations with Litter Size in Two Chinese Indigenous Sheep Breeds

Weimin Wang et al. Int J Mol Sci. .

Abstract

The Small Tailed Han sheep and Hu sheep are two prolific local sheep in China. In this study, the polymorphisms of BMPR-IB (Bone morphogenetic protein receptor IB), BMP-15 (Bone morphogenetic protein 15) and FSHR (follicle stimulating hormone receptor) were investigated to check whether they are associated with litter size in Small Tailed Han sheep and Hu sheep. Consequently, three polymorphisms, FecB mutation in BMPR-IB (c.746A>G), FecG mutation in BMP-15 (c.718C>T) and the mutation (g. 47C>T) in FSHR were found in the above two sheep breeds with a total number of 1630 individuals. The single marker association analysis showed that the three mutations were significantly associated with litter size. The ewes with genotype FecBB/FecBB and FecBB/FecB+ had 0.78 and 0.58 more lambs (p < 0.01) than those with genotype FecB+/FecB+, respectively. The heterozygous Han and Hu ewes with FecXG/FecX+ genotype showed 0.30 (p = 0.05) more lambs than those with the FecX+/FecX+ genotype. For FSHR gene, the ewes with genotype CC had 0.52 (p < 0.01) and 0.75 (p < 0.01) more lambs than those with genotypes TC and TT, respectively. Combined effect analyses indicated an extremely significant interaction (p < 0.01) between the random combinations of BMPR-IB, BMP-15 and FSHR genes on litter size. In addition, the Han and Hu ewes with BB/G+/CC genotype harbor the highest litter size among ewes analyzed in current study. In conclusion, BMPR-IB, BMP-15 and FSHR polymorphisms could be used as genetic markers in multi-gene pyramiding for improving litter size in sheep husbandry.

Keywords: BMP-15 gene; BMPR-IB gene; FSHR gene; Hu sheep; Small Tailed Han sheep; litter size.

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Figures

Figure 1
Figure 1
PCR-RFLP results of different genotypes of the PCR products digested by enzyme Ava IIc.746A>G of ovine BMPR-IB polymorphisms. The genotypes are marked on the top of the lanes. M: DNA Marker (DL2000).
Figure 2
Figure 2
PCR-RFLP results of different genotypes of the PCR products digested by enzyme Hinf I c.718C>T of ovine BMP-15polymorphisms. The genotypes are marked on the top of the lanes. M: DNA Marker (DL2000).
Figure 3
Figure 3
PCR-RFLP results of different genotypes of the PCR products digested by enzyme BsiE I g.47C>T of ovine FSHR polymorphisms. The genotypes are marked on the top of the lanes. M: DNA Marker (DL2000).
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