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. 2015 May 18;15(5):11431-41.
doi: 10.3390/s150511431.

A Colorimetric Enzyme-Linked Immunosorbent Assay (ELISA) Detection Platform for a Point-of-Care Dengue Detection System on a Lab-on-Compact-Disc

Aung Thiha  1   2 , Fatimah Ibrahim  3   4
Affiliations

A Colorimetric Enzyme-Linked Immunosorbent Assay (ELISA) Detection Platform for a Point-of-Care Dengue Detection System on a Lab-on-Compact-Disc

Aung Thiha et al. Sensors (Basel). .

Abstract

The enzyme-linked Immunosorbent Assay (ELISA) is the gold standard clinical diagnostic tool for the detection and quantification of protein biomarkers. However, conventional ELISA tests have drawbacks in their requirement of time, expensive equipment and expertise for operation. Hence, for the purpose of rapid, high throughput screening and point-of-care diagnosis, researchers are miniaturizing sandwich ELISA procedures on Lab-on-a-Chip and Lab-on-Compact Disc (LOCD) platforms. This paper presents a novel integrated device to detect and interpret the ELISA test results on a LOCD platform. The system applies absorption spectrophotometry to measure the absorbance (optical density) of the sample using a monochromatic light source and optical sensor. The device performs automated analysis of the results and presents absorbance values and diagnostic test results via a graphical display or via Bluetooth to a smartphone platform which also acts as controller of the device. The efficacy of the device was evaluated by performing dengue antibody IgG ELISA on 64 hospitalized patients suspected of dengue. The results demonstrate high accuracy of the device, with 95% sensitivity and 100% specificity in detection when compared with gold standard commercial ELISA microplate readers. This sensor platform represents a significant step towards establishing ELISA as a rapid, inexpensive and automatic testing method for the purpose of point-of-care-testing (POCT) in resource-limited settings.

Keywords: Enzyme Linked Immunosorbent Assay (ELISA); Lab-on-Compact Disc; colorimetric sensing; dengue detection; immunosensors; lab-on-a-chip (LOC); optical biosensors; point-of-care testing; smartphone.

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Figures

Figure 1
Figure 1
A general scheme of (a) a Lab-on-Compact Disc (LOCD) ELISA with four sets of assays; (b) a single assay showing reservoirs for various reagents; and (c) antigen coating on the detection chamber substrate with primary and secondary antibody attachment (adapted from [16]). The reagents are sequentially released into the reaction chamber and the end product colour change is detected by an optical sensor.
Figure 2
Figure 2
Block diagram of Colorimetric Enzyme-Linked Immunosorbent Assay (ELISA) detection platform for a Lab-on-a-Compact-Disc (LOCD) System.
Figure 3
Figure 3
(a) Prototype of the LOCD ELISA reader; (b) Smartphone application displaying ELISA test results; (c) sensor operation principle in evaluating absorbance.
Figure 4
Figure 4
Recorded absorbance (OD) over % concentration of sample to calibrate the sensor response.
See this image and copyright information in PMC

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