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. 2015 May;94(20):e813.
doi: 10.1097/MD.0000000000000813.

Peripheral frequency of CD4+ CD28- cells in acute ischemic stroke: relationship with stroke subtype and severity markers

Antonino Tuttolomondo  1 , Rosaria Pecoraro, Alessandra Casuccio, Domenico Di Raimondo, Carmelo Buttà, Giuseppe Clemente, Vittoriano Della Corte, Giuliana Guggino, Valentina Arnao, Carlo Maida, Irene Simonetta, Rosario Maugeri, Rosario Squatrito, Antonio Pinto
Affiliations

Peripheral frequency of CD4+ CD28- cells in acute ischemic stroke: relationship with stroke subtype and severity markers

Antonino Tuttolomondo et al. Medicine (Baltimore). 2015 May.

Erratum in

Abstract

CD4+ CD28- T cells also called CD28 null cells have been reported as increased in the clinical setting of acute coronary syndrome. Only 2 studies previously analyzed peripheral frequency of CD28 null cells in subjects with acute ischemic stroke but, to our knowledge, peripheral frequency of CD28 null cells in each TOAST subtype of ischemic stroke has never been evaluated. We hypothesized that CD4+ cells and, in particular, the CD28 null cell subset could show a different degree of peripheral percentage in subjects with acute ischemic stroke in relation to clinical subtype and severity of ischemic stroke.The aim of our study was to analyze peripheral frequency of CD28 null cells in subjects with acute ischemic stroke in relation to TOAST diagnostic subtype, and to evaluate their relationship with scores of clinical severity of acute ischemic stroke, and their predictive role in the diagnosis of acute ischemic stroke and diagnostic subtypeWe enrolled 98 consecutive subjects admitted to our recruitment wards with a diagnosis of ischemic stroke. As controls we enrolled 66 hospitalized patients without a diagnosis of acute ischemic stroke. Peripheral frequency of CD4+ and CD28 null cells has been evaluated with a FACS Calibur flow cytometer.Subjects with acute ischemic stroke had a significantly higher peripheral frequency of CD4+ cells and CD28 null cells compared to control subjects without acute ischemic stroke. Subjects with cardioembolic stroke had a significantly higher peripheral frequency of CD4+ cells and CD28 null cells compared to subjects with other TOAST subtypes. We observed a significant relationship between CD28 null cells peripheral percentage and Scandinavian Stroke Scale and NIHSS scores. ROC curve analysis showed that CD28 null cell percentage may be useful to differentiate between stroke subtypes.These findings seem suggest a possible role for a T-cell component also in acute ischemic stroke clinical setting showing a different peripheral frequency of CD28 null cells in relation of each TOAST subtype of stroke.

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Conflict of interest statement

There are no funding and conflicts of interest to disclose.

Figures

FIGURE 1
FIGURE 1
ROC curves of CD4+ and CD28− cells cutoff values toward diagnosis of ischemic stroke.
FIGURE 2
FIGURE 2
ROC curves of CD4+ and CD28− cells cutoff values toward diagnosis of cardioembolic subtype of ischemic stroke.
FIGURE 3
FIGURE 3
ROC curves of CD4+ and CD28− cells cutoff values toward diagnosis of LAAS subtype of ischemic stroke.
See this image and copyright information in PMC

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