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. 2015 May 23:14:29.
doi: 10.1186/s12941-015-0086-0.

Antibacterial activity of native California medicinal plant extracts isolated from Rhamnus californica and Umbellularia californica

Maria G Carranza  1 , Mary B Sevigny  2 , Debashree Banerjee  3 , Lacie Fox-Cubley  4
Affiliations

Antibacterial activity of native California medicinal plant extracts isolated from Rhamnus californica and Umbellularia californica

Maria G Carranza et al. Ann Clin Microbiol Antimicrob. .

Abstract

Background: Antimicrobial resistance (AMR) is a major threat to global public health. Medicinal plants have long been used as remedies for infectious diseases by native cultures around the world and have the potential for providing effective treatments for antibiotic-resistant infections. Rhamnus californica (Rhamnaceae) and Umbellularia californica (Lauraceae) are two indigenous California plant species historically used by Native Americans to treat skin, respiratory and gastrointestinal infections. This study aimed to assess the in vitro antimicrobial activity of methanolic extracts of leaves and bark of R. and U. californica against methicillin-resistant Staphylococcus aureus (MRSA) and other Gram-positive and Gram-negative bacteria.

Methods: Methanolic extracts of leaves and bark of R. and U. californica were prepared by soxhlet extraction and evaluated for their antimicrobial activity against Bacillus cereus, Streptococcus pyogenes, Mycobacterium smegmatis, Staphylococcus aureus, MRSA, Escherichia coli and Pseudomonas aeruginosa using disc diffusion and minimal inhibitory concentration (MIC) assays. Chemical profiling of the extracts was performed using standard methods.

Results: All extracts inhibited the growth of MRSA and other Gram-positive bacteria with MICs of 3.3-6.0 mg/ml. Gram-negative organisms were unaffected by these extracts. U. californica extracts (leaves and bark) had the lowest MIC values. Chemical profiling detected the presence of quinones, alkaloids, flavonoids, cardenolides, tannins and saponins in these extracts. Our study is the first to report the antimicrobial properties of R. and U. californica and illustrates their promising anti-MRSA potential.

Conclusions: Our results give scientific credence to the traditional medicinal uses of these plants by the indigenous peoples of California. Further investigation of the secondary metabolites responsible for the antimicrobial activity of these extracts against MRSA is warranted.

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Figures

Fig. 1
Fig. 1
Selected examples of reported structural classes from R. californica and U. californica
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References

    1. Klevens RM, Morrison MA, Nadle J, Petit S, Gershman K, Ray S, et al. Invasive methicillin-resistant Staphylococcus aureus infections in the United States. JAMA. 2007;298(15):1763–71. doi: 10.1001/jama.298.15.1763. - DOI - PubMed
    1. World Health Organization (WHO). Antimicrobial Resistance. In: Fact Sheet No 194. 2014. http://www.who.int/mediacentre/factsheets/fs194/en/. Accessed 07 Aug 2014.
    1. Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001;109(Suppl 1):69–75. doi: 10.1289/ehp.01109s169. - DOI - PMC - PubMed
    1. Moerman DE. Native American medicinal plant : an ethnobotanical dictionary. Portland, Or: Timber Press; 2009.
    1. O’Neill T, Johnson JA, Webster D, Gray CA. The Canadian medicinal plant Heracleum maximum contains antimycobacterial diynes and furanocoumarins. J Ethnopharmacol. 2013;147(1):232–7. doi: 10.1016/j.jep.2013.03.009. - DOI - PubMed

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