Association of the angiopoietin-like protein 8 rs2278426 polymorphism and several environmental factors with serum lipid levels

Abstract

The present study was performed to examine the association of the angiopoietin-like protein 8 (ANGPTL8) rs2278426 single nucleotide polymorphism (SNP) and several environmental factors with serum lipid profiles in the Mulao and Han populations. A total of 879 individuals of the Mulao ethnic group and 865 individuals of the Han Chinese ethnic group were included. The serum apolipoprotein (Apo) B level was higher, however the serum ApoA1 level was lower in the Mulao individuals than in the Han individuals (P<0.05 and P<0.001, respectively). The genotypic and allelic frequencies, and the association with the ANGPTL8 rs2278426 SNP were different between the Mulao and Han populations. The frequency of the A allele was 17.80% in Han individuals and 23.04% in Mulao individuals (P<0.001). The frequencies of GG, GA and AA genotypes were 68.79, 26.82 and 4.39% in the Han population, and 60.64, 32.65 and 6.71% in the Mulao population (P<0.005), respectively. A significant association between the SNP and serum lipid traits was only detected in Han females and not in Han males or in the Mulao population. The subjects with GA/AA genotypes had lower low‑density lipoprotein cholesterol (LDL‑C) and ApoB levels, and higher ApoA1 levels with a higher ApoA1/ApoB ratio than the subjects with the GG genotype in the Han population. Subgroup analyses revealed that the subjects with the GA/AA genotype had lower levels of total cholesterol, LDL‑C and ApoB, and a higher ApoA1/ApoB ratio than the subjects with the GG genotype in Han females (P<0.05‑P<0.001). Serum lipid parameters were also associated with several environmental factors, including dietary patterns, lifestyle, obesity, physical inactivity and hypertension, in the two ethnic groups (P<0.05‑0.001). These findings suggest that there may be an ethnic‑ and gender‑specific association of the rs2278426 SNP and serum lipid parameters.

Figure 1

Electrophoresis of polymerase chain reaction products of the samples. Lane M is the 100 bp marker ladder; Lane 1–5 are samples, the 387 bp bands are the target genes.

Figure 2

Genotyping of the angiopoietin-like protein 8 rs2278426 SNP. Lane M is the 100 bp marker Ladder; Lanes 1 and 2, GG genotype (387 bp); Lanes 3 and 4, AG genotype (387, 331 and 56 bp); and lanes 5 and 6, AA genotype (331 and 56 bp). The 56 bp segment is not visible in the gel owing to its fast migration speed.

Figure 3

Nucleotide reverse sequence for part of angiopoietin-like protein 8 rs2278426. (A) GG genotype; (B) GA genotype; (C) AA genotype.