Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration

Abstract

Bacterial cellulose (BC) and silk fibroin (SF) are natural biopolymers successfully applied in tissue engineering and biomedical fields. In this work nanocomposites based on BC and SF were prepared and characterized by scanning electron microscopy (SEM), infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). In addition, the investigation of cytocompatibility was done by MTT, XTT and Trypan Blue dye technique. Cellular adhesion and proliferation were detected additionally. The evaluation of genotoxicity was realized by micronucleus assay. In vitro tests showed that the material is non-cytotoxic or genotoxic. SEM images revealed a greater number of cells attached at the BC/SF:50% scaffold surface than the pure BC one, suggesting that the presence of fibroin improved cell attachment. This could be related to the SF amino acid sequence that acts as cell receptors facilitating cell adhesion and growth. Consequently, BC/SF:50% scaffolds configured an excellent option in bioengineering depicting its potential for tissue regeneration and cultivation of cells on nanocomposites.

Keywords: Bacterial cellulose; Biocompatible materials; MTT (PubChem CID: 64965); Nanocomposites; Scaffold; Silk fibroin; Tissue engineering.; XTT (PubChem CID: 14195569); d-Glucose (PubChem CID: 5793); glycine (PubChem CID: 750); l-Alanine (PubChem CID: 5950).