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. 2015 May 6:9:2545-51.
doi: 10.2147/DDDT.S82347. eCollection 2015.

Sonodynamic effect of hematoporphyrin monomethyl ether on ligature-induced periodontitis in rats

Deshu Zhuang  1 , Jialong Han  1 , Liangjia Bi  1 , Yueping Wang  1 , Yanru Hao  2 , Qi Zhou  3 , Wenwu Cao  4
Affiliations

Sonodynamic effect of hematoporphyrin monomethyl ether on ligature-induced periodontitis in rats

Deshu Zhuang et al. Drug Des Devel Ther. .

Erratum in

Abstract

Objectives: The aim of this study was to perform a histological evaluation of sonodynamic therapy (SDT) of hematoporphyrin monomethyl ether (HMME) on artificially induced periodontal disease in rats.

Methods: Submerging ligatures were placed at the subgingival region of the first maxillary molar in rats. Eighty rats were randomly assigned into four groups: group 1 received no treatment; group 2 was subjected to 50 μg/mL HMME alone; group 3 was treated with low-intensity ultrasound alone (1 W/cm(2)); and group 4 was treated with 50 μg/mL HMME plus ultrasound irradiation (1 MHz, 30 minutes). Ten rats in each group were euthanized at 7 and 15 days, and periodontal tissue samples were taken for histological examination.

Results: The animals treated by SDT showed less bone loss (P<0.05) at all experimental periods than the other three groups. No significant differences were found between the control and HMME groups (P>0.05).

Conclusion: Our results suggest that HMME-mediated SDT can effectively alleviate the periodontal tissue destruction in artificially induced periodontitis in rats. Hence, SDT may have good clinic potential as a noninvasive treatment of periodontal diseases.

Keywords: alveolar bone loss; animal studies; histology; periodontal tissues; sonodynamic therapy.

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Figures

Figure 1
Figure 1
Schematic diagram of the ultrasound system.
Figure 2
Figure 2
Photomicrographs of bone tissue in the furcation region of the mandibular first molar with induced periodontitis. Notes: (A) Untreated control group at 15 days. (B) HMME group at 15 days. (C) Ultrasound group at 15 days. (D) Sonodynamic therapy group at 15 days. Hematoxylin and eosin staining; original magnification for A, B, C, and D was ×12.5. Abbreviation: HMME, hematoporphyrin monomethyl ether.
Figure 3
Figure 3
Photomicrographs of bone tissue in the furcation region of the mandibular first molar with induced periodontitis. Notes: (A) Untreated control group at 15 days. (B) Sonodynamic therapy group at 15 days. Masson trichrome staining; original magnification for A and B was ×12.5.
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