Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jan;12(1):1-10.

Antimicrobial effect of zataria multiflora extract in comparison with chlorhexidine mouthwash on experimentally contaminated orthodontic elastomeric ligatures

Hossein Aghili  1 , Abbas Ali Jafari Nadoushan  2 , Vahid Herandi  3
Affiliations

Antimicrobial effect of zataria multiflora extract in comparison with chlorhexidine mouthwash on experimentally contaminated orthodontic elastomeric ligatures

Hossein Aghili et al. J Dent (Tehran). 2015 Jan.

Abstract

Objectives: Long-term use of orthodontic appliances and fixation ligatures creates a favorable environment for the accumulation of oral normal microflora and increases the risk of enamel demineralization and periodontal disease. The aim of this study was to compare the antimicrobial effects of Zataria Multiflora extract and 0.2% chlorhexidine (CHX) mouthwash on experimentally contaminated orthodontic elastomeric ligatures.

Materials and methods: In this lab trial study, Iranian and foreign-made elastomeric ligatures were experimentally contaminated in Streptococcus mutans, Enterococcus faecalis and Candida albicans suspensions. Ligatures were then decontaminated using 0.2% CHX as the control, 0.5 mg/ml Zataria multiflora extract mouthwashes as the test and phosphate buffered saline (PBS) as the negative control for one hour. Antimicrobial properties of both solutions were evaluated by comparing the mean viable bacterial cell count on both rings after decontamination, using SPSS version 15 software.

Results: The mean viable bacterial cell count on Iranian ligatures was greater than that on foreign-made ligatures before disinfection (P=0.001), however this difference for C. albicans was not statistically significant (P=0.061). Chlorhexidine mouthwash completely eliminated all tested microorganisms attached to both elastomeric rings, but Zataria extract was only capable of completely eliminating C. albicans from both ligatures. Statistically significant differences were found in viable bacterial counts on both ligatures before and after disinfection with Zataria extract (P=0.0001).

Conclusion: Zataria multiflora extract has antimicrobial properties and can be used for disinfection of elastomeric ligatures. In vivo studies are required to evaluate the efficacy of the incorporation of this herbal extract in mouthwashes for orthodontic patients.

Keywords: Candida albicans; Chlorhexidine; Enterococcus faecalis; Streptococcus mutans; Zataria multiflora.

PubMed Disclaimer

Figures

Fig 1
Fig 1
Left: Washing of contaminated ligatures; Right: S. mutans colonies isolated from ligatures
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Sallum EJ, Nouer DF, Klein MI, Gonçalves RB, Machion L, Sallum AW, et al. Clinical and microbiologic changes after removal of orthodontic appliances. Am J Orthod Dentofacial Orthop. 2004; 126: 363– 366. - PubMed
    1. Lee SM, Yoo SY, Kim HS, Kim KW, Yoon YJ, Lim SH, et al. Prevalence of putative periodontopathogens in subgingival dental plaques from gingivitis lesions in Korean orthodontic patients. J Microbiol. 2005; 43: 260– 265. - PubMed
    1. Sukontapatipark W, el-Agroudi MA, Selliseth NJ, Thunold K, Selvig KA.Bacterial colonization associated with fixed orthodontic appliances. A scanning electron microscopy st udy. Eur J Orthod. 2001; 23: 475– 484. - PubMed
    1. Borzabadi-farahani A, Eslamipour F.Orthodontic treatment needs in an urban Iranian population, an epidemiological study of 11–14 year old children. Euro J Pedi Dent 2009; 10( 2): 69– 75. - PubMed
    1. Hagg U, Kaveewatcharanont P, Samaranayake YH, Samaranayake L.The effect of fixed orthodontic appliances on the oral carriage of Candida species and Enterobacteriaceae. Eur J Orthod 2004; 26: 623– 629. - PubMed

LinkOut - more resources