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. 2015 May 22;7(5):1808-20.
doi: 10.3390/toxins7051808.

Influence of honeybee sting on peptidome profile in human serum

Jan Matysiak  1 , Agata Światły  2 , Joanna Hajduk  3 , Joanna Matysiak  4 , Zenon J Kokot  5
Affiliations

Influence of honeybee sting on peptidome profile in human serum

Jan Matysiak et al. Toxins (Basel). .

Abstract

The aim of this study was to explore the serum peptide profiles from honeybee stung and non-stung individuals. Two groups of serum samples obtained from 27 beekeepers were included in our study. The first group of samples was collected within 3 h after a bee sting (stung beekeepers), and the samples were collected from the same person a second time after at least six weeks after the last bee sting (non-stung beekeepers). Peptide profile spectra were determined using MALDI-TOF mass spectrometry combined with Omix, ZipTips and magnetic beads based on weak-cation exchange (MB-WCX) enrichment strategies in the mass range of 1-10 kDa. The samples were classified, and discriminative models were established by using the quick classifier, genetic algorithm and supervised neural network algorithms. All of the statistical algorithms used in this study allow distinguishing analyzed groups with high statistical significance, which confirms the influence of honeybee sting on the serum peptidome profile. The results of this study may broaden the understanding of the human organism's response to honeybee venom. Due to the fact that our pilot study was carried out on relatively small datasets, it is necessary to conduct further proteomic research of the response to honeybee sting on a larger group of samples.

Keywords: MALDI; honeybee venom; peptidome profiling; sting.

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Figures

Figure 1
Figure 1
Average MALDI-TOF spectra of serum samples of stung (red) and non-stung (green) individuals pretreated with Omix over the full scan range of m/z 1–10 kDa (A); zoomed spectra over the m/z 1–2.5 kDa range (B); zoomed spectra over the m/z 2.5–5.0 kDa range (C); zoomed spectra over the m/z 5–7.5 kDa range (D); zoomed spectra over the m/z 7.5–10 kDa range (E).
Figure 2
Figure 2
Average MALDI-TOF spectra of serum samples of stung (red) and non-stung (green) individuals pretreated with ZipTips over the full scan range of m/z 1–10 kDa (A); zoomed spectra over the m/z 1–2.5 kDa range (B); zoomed spectra over the m/z 2.5–5.0 kDa range (C); zoomed spectra over the m/z 5–7.5 kDa range (D); zoomed spectra over the m/z 7.5–10 kDa range (E).
Figure 3
Figure 3
Average MALDI-TOF spectra of serum samples of stung (red) and non-stung (green) individuals pretreated with magnetic beads based on weak-cation exchange (MB-WCX) over the full scan range of m/z 1–10 kDa (A); zoomed spectra over the m/z 1–2.5 kDa range (B); zoomed spectra over the m/z 2.5–5.0 kDa range (C); zoomed spectra over the m/z 5–7.5 kDa range (D); zoomed spectra over the m/z 7.5–10 kDa range (E).
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