Winter Polycyclic Aromatic Hydrocarbon-Bound Particulate Matter from Peri-urban North China Promotes Lung Cancer Cell Metastasis

Abstract

On the basis of the close relationship between human exposure to high concentrations of small particulate matter (PM) and increased lung cancer mortality, PM was recently designated as a Group I carcinogen. Considering that PM is highly heterogeneous, the potential health risks of PM promoting tumor metastasis in lung cancer, as well as its chemical characteristics, remain elusive. In the present study, we collected PM2.5 and PM10 in a peri-urban residential site of Taiyuan and determined the concentration and source of polycyclic aromatic hydrocarbons (PAHs). The results indicated that 18 PAHs, ranging from 38.21 to 269.69 ng/m(3) (for PM2.5) and from 44.34 to 340.78 ng/m(3) (for PM10), exhibited seasonal variations, and the PAHs in winter PM mainly originated from coal combustion. We calculated the benzo(a)pyrene-equivalent (BaPeq) and found that the PAH-bound PM in winter exhibited higher carcinogenic risks for humans. Following this result, in vitro bioassays demonstrated that PM2.5 and PM10 induced A549 cell migration and invasion, and the mechanism involved reactive oxygen species (ROS)-mediated epithelial-to-mesenchymal transition (EMT) activation and extracellular matrix (ECM) degradation. Our data indicate the potential risk for winter PAH-bound PM from peri-urban North China promoting lung cancer cell metastasis and reveal a mechanistic basis for treating, ameliorating, or preventing outcomes in polluted environments.