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. 2014 Mar 4;28(2):301-305.
doi: 10.1080/13102818.2014.909171. Epub 2014 Jul 8.

Differentiation of mixed biological traces in sexual assaults using DNA fragment analysis

Аleksandar Apostolov  1
Affiliations

Differentiation of mixed biological traces in sexual assaults using DNA fragment analysis

Аleksandar Apostolov. Biotechnol Biotechnol Equip. .

Abstract

During the investigation of sexual abuse, it is not rare that mixed genetic material from two or more persons is detected. In such cases, successful profiling can be achieved using DNA fragment analysis, resulting in individual genetic profiles of offenders and their victims. This has led to an increase in the percentage of identified perpetrators of sexual offenses. The classic and modified genetic models used, allowed us to refine and implement appropriate extraction, polymerase chain reaction and electrophoretic procedures with individual assessment and approach to conducting research. Testing mixed biological traces using DNA fragment analysis appears to be the only opportunity for identifying perpetrators in gang rapes.

Keywords: DNA identification; Y chromosome; material evidence; mixed biological traces; sexual abuse.

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Figures

Figure 1.
Figure 1.
Mixed traces of semen from two men. Sample 3: Sizer 50–500. Samples 30, 31 and 32: a study on Y chromosome marker DYS392 with sequencing – semen on sports clothes and two suspects. Samples 33 and 34: a study on Y chromosome markers DYS393 and DYS390 with sequencing – two suspects, and sample 35: mixed semen from two men on physical evidence. Samples 30 and 35: mixed biological material where differentiation of the DNA profiles of the two persons was possible.
Figure 2.
Figure 2.
Mixed traces of semen – Y-chromosome exclusion of complicity. Sample 9: allele witness using autosomal markers TH01 and FES. Samples 17–22: testing using autosomal marker TH01 – vaginal smear, briefs, the victim and three suspects. Samples 17 and 19: mixed biological material with no potential for the differentiation of the DNA profiles. Samples 23–27: testing of biological material from four suspects, using Y chromosome markers DYS393 and DYS390, with a potential for the exclusion of a suspect as the perpetrator – sample 27.
Figure 3.
Figure 3.
Mixed traces – semen from a man, in the vaginal contents. Sample 1: Sizer 50–500. Samples 29, 31 and 33: tested using an autosomal marker CYAR04 with sequence – vaginal smear, suspect and victim. Sample 29: mixed biological material with the potential for differentiation of the DNA profiles of the two parties.
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