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. 2015 Mar 4;29(2):309-314.
doi: 10.1080/13102818.2015.1004899. Epub 2015 Jan 29.

Assessment of genetic characteristics of Aconitum germplasms in Xinjiang Province (China) by RAPD and ISSR markers

Feicui Zhao  1 , Jihong Nie  2 , Muzhi Chen  2 , Guirong Wu  3
Affiliations

Assessment of genetic characteristics of Aconitum germplasms in Xinjiang Province (China) by RAPD and ISSR markers

Feicui Zhao et al. Biotechnol Biotechnol Equip. .

Abstract

Aconitum is a medicinal treasure trove that grows extensively on fertile pastures in Xinjiang Province (China); however, its molecular genetic characteristics are still poorly studied. We studied Aconitum kusnezoffii Reichb., Aconitum soongaricum Stapf., Aconitum carmichaelii Debx. and Aconitum leucostomum Worosch, using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) techniques, to evaluate their genetic relationship and potential medicinal value. Our results showed that A.kusnezoffii Reichb. and A.soongaricum Stapf. have close genetic relationship and cluster together. Polymorphism rates of 97.25% and 98.92% were achieved by using 15 RAPD and 15 ISSR primers, respectively. Based on Nei's gene diversity (H) and Shannon's index (I), the inter-population diversity (Hs ) was higher when compared with the intra-population diversity (Hp ). Among the three Aconitum populations, the coefficient of gene differentiation (Gst ) was 0.4358 when evaluated by RAPD and 0.5005 by ISSR. The genetic differentiation among the three Aconitum populations was highly significant, suggesting low gene flow (Nm ). This was confirmed by the estimates of gene flow (Nm = 0.6473 and Nm = 0.4991, based on ISSR and RAPD data, respectively). Comparing the RAPD and ISSR results, the two DNA markers proved similarly effective in the assessment of the genetic characteristics of the studied Aconitum populations and could be used for reliable fingerprinting and mapping in studies on Aconitum diversity in view of Aconitum suitability for development and protection.

Keywords: Aconitum; ISSR; RAPD; genetic characteristics.

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Figures

Figure 1.
Figure 1.
RAPD electrophoretic analysis of 15 Aconitum specimens (a: S112 primer; b: S41 primer). M: DL2000 DNA marker: 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 2000 bp.
Figure 2.
Figure 2.
ISSR electrophoretic analysis of 15 Aconitum specimens (a: UBC881 primer; b: UBC823 primer). M: DL2000 DNA marker: 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 2000 bp.
Figure 3.
Figure 3.
Dendrogram plot of 15 Aconitum specimens by UPGMA cluster analysis (RAPD).
Figure 4.
Figure 4.
Dendrogram plot of 15 Aconitum specimens by UPGMA cluster analysis (ISSR).
See this image and copyright information in PMC

References

    1. Anderson JA. Churchill GA. Autrique JE. Tanksley SD. Sorrells ME. Optimizing parental selection for genetic linkage maps. Genome. 1993;36:181–186. - PubMed
    1. Archak S. Ambika B. Gaikwad D. Gautam EV. Rao VB. Swamy KRM. Karihaloo JL. DNA fingerprinting of India cashew (Anacardium occidentale L.) varieties using RAPD and ISSR techniques. Euphytica. 2003;5:397–404.
    1. Brijesh S. Daswani PG. Tetali P. Studies on Pongamia pinnate (L.) Pierre leaves: understanding the mechanisms of action in infectious diarrhea. J Zhejiang Univ Sci B. 2006;7:665–674. - PMC - PubMed
    1. Liu SM. Nie JH. Pan R. Zhao FC. Determination of the content of total alkaloid in Xinjiang genus Aconitum. J Xinjiang Med Univ. 2012;4:1009–5551.
    1. Bussell JD. The distribution of random amplified polymorphic DNA (RAPD) diversity among populations of Isotoma petraea (Lobeliaceae) Mol Ecol. 1999;8:775–789.

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