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. 2015 Jun;52(6):3803-11.
doi: 10.1007/s13197-014-1449-9. Epub 2014 Jun 19.

Subcritical water extraction and antioxidant activity evaluation with on-line HPLC-ABTS(·+) assay of phenolic compounds from marigold (Tagetes erecta L.) flower residues

Honggao Xu  1 , Weiyou Wang  1 , Junping Jiang  1 , Fang Yuan  1 , Yanxiang Gao  1
Affiliations

Subcritical water extraction and antioxidant activity evaluation with on-line HPLC-ABTS(·+) assay of phenolic compounds from marigold (Tagetes erecta L.) flower residues

Honggao Xu et al. J Food Sci Technol. 2015 Jun.

Abstract

Subcritical water extraction (SWE) of phenolics was investigated from marigold (Tagetes erecta L.) flower residues. The total phenolics content (TPC), total flavonoids content (TFC) and antioxidant capacities of extracts were determined, furthermore, antioxidant activities of individual compounds were evaluated with on-line HPLC-ABTS(•+) system. The optimum SWE time was 45 min, solid-to-liquid ratio was 1:50, and the highest TPC and TFC were obtained at 220 °C respectively. The effect of SWE temperature on TPC and TFC was significant (p < 0.05), and TPC was ranged from 28.42 ± 0.94 to 124.27 ± 1.94 (mg GAE/g), and TFC ranged from 34.21 ± 0.36 to 133.22 ± 1.57 (mg GAE/g) between 80 and 220 °C. On-line HPLC-ABTS(•+) profiles revealed that quercetagetin from SWE at 200 °C had nearly twofold radical scavenging activities than that by leaching extraction.

Keywords: Antioxidant activity; Marigold flower residues; On-line HPLC-ABTS·+; Phenolic compounds; Subcritical water extraction (SWE).

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Figures

Fig. 1
Fig. 1
Effect of different extraction parameters on TPC and TFC in the extracts from marigold flower residues: (a), temperature, (b) solid-to-liquid ratio and (c) extraction time
Fig. 2
Fig. 2
Effect of different extraction temperatures on total antioxidant capacity of the extracts from marigold flower residues
Fig. 3
Fig. 3
Effect of different extraction temperatures on A280 and A420 of the extracts from marigold flower residues
Fig. 4
Fig. 4
Effect of different extraction temperatures on 5-HMF accumulation in the extracts from marigold flower residues
Fig. 5
Fig. 5
Effect of different extraction temperatures on TRS and TAA contents in the extracts from marigold flower residues
Fig. 6
Fig. 6
On-line HPLC-ABTS•+ chromatographic profiles of extracts obtained by leaching with 60 % acetone solution and SWE at 200 °C
See this image and copyright information in PMC

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