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. 2015 Jul;12(7):637-40.
doi: 10.1038/nmeth.3411. Epub 2015 Jun 1.

Functional differentiation of human pluripotent stem cells on a chip

Giovanni G Giobbe  1 , Federica Michielin  1 , Camilla Luni  1 , Stefano Giulitti  1 , Sebastian Martewicz  1 , Sirio Dupont  2 , Annarosa Floreani  3 , Nicola Elvassore  1
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Functional differentiation of human pluripotent stem cells on a chip

Giovanni G Giobbe et al. Nat Methods. 2015 Jul.

Abstract

Microengineering human "organs-on-chips" remains an open challenge. Here, we describe a robust microfluidics-based approach for the differentiation of human pluripotent stem cells directly on a chip. Extrinsic signal modulation, achieved through optimal frequency of medium delivery, can be used as a parameter for improved germ layer specification and cell differentiation. Human cardiomyocytes and hepatocytes derived on chips showed functional phenotypes and responses to temporally defined drug treatments.

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