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. 2015 Apr 18;8(4):e17920.
doi: 10.5812/jjm.8(4)2015.17920. eCollection 2015 Apr.

Expression Profiles of TGF-β and TLR Pathways in Porphyromonas gingivalis and Prevotella intermedia Challenged Osteoblasts

Kubra Aydin  1 , Fatma Yesim Ekinci  1 , May Korachi  1
Affiliations

Expression Profiles of TGF-β and TLR Pathways in Porphyromonas gingivalis and Prevotella intermedia Challenged Osteoblasts

Kubra Aydin et al. Jundishapur J Microbiol. .

Abstract

Background: The presence of certain oral pathogens at implant sites can hinder the osseointegration process. However, it is unclear how and by what microorganisms it happens.

Objectives: This study investigated whether the presence of oral pathogens of Porphyromonas gingivalis and Prevotella intermedia individually, play a role in the failure of bone formation by determining the expression profiles of Transforming Growth Factor Beta (TGF-β/Bone Morphogenic Protein (BMP) and Toll-Like Receptor (TLR) pathways in challenged osteoblasts.

Materials and methods: Cell viability of P. gingivalis and P. intermedia challenged osteoblasts were determined by WST assay. Changes in osteoblast morphology and inhibition of mineralization were observed by Scanning Electron Microscopy (SEM) and Von Kossa staining, respectively. Expression of TGF-β and TLR pathway genes on challenged cells were identified by RT profiler array. Both P. gingivalis and P. intermedia challenges resulted in reduced viability and mineralization of osteoblasts.

Results: Viability was reduced to 56.8% (P. gingivalis) and 52.75% (P. intermedia) at 1000 multiplicity. Amongst 48 genes examined, expressions of BMPER, SMAD1, IL8 and NFRKB were found to be highly upregulated by both bacterial challenges (Fold Change > 4).

Conclusions: P. gingivalis and P. intermedia could play a role in implant failure by changing the expression profiles of genes related to bone formation and resorption.

Keywords: Bone Formation; Osseointegration; Receptors Transforming Growth Factor; Receptors, Toll Like.

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Figures

Figure 1.
Figure 1.. Scanning Electron Microscopy Images of Challenged Osteoblasts by Porphyromonas gingivalis and Prevotella intermedia at a MOI of 1000 for 2 Hours
Figure 2.
Figure 2.. Relative Gene Expression (Fold Change) was Determined by Real-Time PCR Using Specific Primers to ALP and BGN Genes Following Bacterial Challenges
The gene expression of Porphyromonas gingivalis challenged osteoblasts is shown as black bars. The gene expression of Prevotella intermedia challenged osteoblasts is shown as grey bars.
See this image and copyright information in PMC

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