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Clinical Trial
. 2015 Aug;35(8):1237-40.
doi: 10.1038/jcbfm.2015.119. Epub 2015 Jun 3.

The fatty acid amide hydrolase C385A variant affects brain binding of the positron emission tomography tracer [11C]CURB

Isabelle Boileau  1 , Rachel F Tyndale  2 , Belinda Williams  3 , Esmaeil Mansouri  3 , Duncan J Westwood  4 , Bernard Le Foll  5 , Pablo M Rusjan  6 , Romina Mizrahi  7 , Vincenzo De Luca  8 , Qian Zhou  9 , Alan A Wilson  10 , Sylvain Houle  10 , Stephen J Kish  11 , Junchao Tong  12
Affiliations
Clinical Trial

The fatty acid amide hydrolase C385A variant affects brain binding of the positron emission tomography tracer [11C]CURB

Isabelle Boileau et al. J Cereb Blood Flow Metab. 2015 Aug.

Abstract

The common functional single-nucleotide polymorphism (rs324420, C385A) of the endocannabinoid inactivating enzyme fatty acid amide hydrolase (FAAH) has been associated with anxiety disorder relevant phenotype and risk for addictions. Here, we tested whether the FAAH polymorphism affects in vivo binding of the FAAH positron emission tomography (PET) probe [(11)C]CURB ([(11)C-carbonyl]-6-hydroxy-[1,10-biphenyl]-3-yl cyclohexylcarbamate (URB694)). Participants (n=24) completed one [(11)C]CURB/PET scan and were genotyped for rs324420. Relative to C/C (58%), A-allele carriers (42%) had 23% lower [(11)C]CURB binding (λk3) in brain. We report evidence that the genetic variant rs324420 in FAAH is associated with measurable differences in brain FAAH binding as per PET [(11)C]CURB measurement.

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Figures

Figure 1
Figure 1
Scattergram of [11C]CURB ([11C-carbonyl]-6-hydroxy-[1,10-biphenyl]-3-yl cyclohexylcarbamate (URB694)) λk3 in A/A+A/C and CC homozygotes for the fatty acid amide hydrolase C385A variants. *Significant at P<0.05; §P=0.1.
See this image and copyright information in PMC

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