doi: 10.1093/nar/5.4.1403.
Short RNA chains synthesized at low pH are initiated at promoter sites
- PMID: 26044
- PMCID: PMC342087
- DOI: 10.1093/nar/5.4.1403
Item in Clipboard
Short RNA chains synthesized at low pH are initiated at promoter sites
Nucleic Acids Res.
1978 Apr.
Free PMC article
Abstract
Under non optimal conditions- either with limiting substrate concentrations (1) or at low pH (2)- RNA polymerase of Escherichia coli synthesizes very short RNA chains. By sequencing one RNA species synthesized at pH 5.8 upon T7 DNA we were able to demonstrate that under these conditions transcription is initiated at a normal promoter site (here A1) but however is terminated soon afterwards at specific artificial sites not used in vivo.
Similar articles
-
Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.Proc Natl Acad Sci U S A. 1975 Mar;72(3):784-8. doi: 10.1073/pnas.72.3.784. Proc Natl Acad Sci U S A. 1975. PMID: 1093168 Free PMC article.
-
T7 early RNAs are generated by site-specific cleavages.Proc Natl Acad Sci U S A. 1973 May;70(5):1559-63. doi: 10.1073/pnas.70.5.1559. Proc Natl Acad Sci U S A. 1973. PMID: 4576024 Free PMC article.
-
The effect of various templates and oligonucleotide primers on RNA and poly (A) synthesis by E. coli and T7 RNA polymerases.FEBS Lett. 1978 Feb 1;86(1):108-12. doi: 10.1016/0014-5793(78)80109-4. FEBS Lett. 1978. PMID: 340279 No abstract available.
-
Interaction of oligoribocytidylates with T7 DNA in neutral and acid media.Biochemistry. 1977 Dec 13;16(25):5414-20. doi: 10.1021/bi00644a002. Biochemistry. 1977. PMID: 21684
-
Regulation of RNA metabolism of T7 and related phages.Annu Rev Genet. 1972;6:191-202. doi: 10.1146/annurev.ge.06.120172.001203. Annu Rev Genet. 1972. PMID: 4581486 Review. No abstract available.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
