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. 2015:1312:419-26.
doi: 10.1007/978-1-4939-2694-7_42.

B-Cell ELISPOT: For the Identification of Antigen-Specific Antibody-Secreting Cells

Hemangi B Shah  1 , Kristi A Koelsch
Affiliations

B-Cell ELISPOT: For the Identification of Antigen-Specific Antibody-Secreting Cells

Hemangi B Shah et al. Methods Mol Biol. 2015.

Abstract

The B-cell ELISPOT assay is a sensitive tool that can be utilized to measure total immunoglobulin (Ig) and antigen-specific antibody-secreting cells. Typically, membrane-bound antigen enables binding of antibody secreted by B-cells. Bound antibody is then detected by using an anti-Ig antibody and a colorimetric substrate, resulting in colored spots on the membrane that can be easily enumerated. Here we have described a method to quantitate antigen-specific antibody-secreting cells from the spleen or bone marrow of a vaccinated mouse.

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