Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015:1312:427-34.
doi: 10.1007/978-1-4939-2694-7_43.

T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells

Kerry M Leehan  1 , Kristi A Koelsch
Affiliations

T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells

Kerry M Leehan et al. Methods Mol Biol. 2015.

Abstract

The ELISPOT is a powerful functional assay used to detect biological activity and immunological secretions from immune cells. In this chapter, we specifically discuss T cell ELISPOT methods for the detection of secreted cytokines. A detailed protocol is given enabling the detection of interferon gamma-secreting CD8(+) T cells and/or peripheral blood mononuclear cells following their isolation and polyclonal activation. Included is a brief discussion on choosing the activation method for your T cell ELISPOT assay, as well as additional instructions for the adaptation of this protocol for the study of memory and antigen-specific T cell responses.

PubMed Disclaimer

MeSH terms

Substances

LinkOut - more resources