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. 2015:1312:481-5.
doi: 10.1007/978-1-4939-2694-7_50.

Fingerprint deposition on nitrocellulose and polyvinylidene difluoride membranes using alkaline phosphatase

Biji T Kurien  1 , Debashish Danda, R Hal Scofield
Affiliations

Fingerprint deposition on nitrocellulose and polyvinylidene difluoride membranes using alkaline phosphatase

Biji T Kurien et al. Methods Mol Biol. 2015.

Abstract

Dactyloscopy or fingerprint identification is a vital part of forensic evidence. Identification with fingerprints has been known since the finding of finger impressions on the clay surface of Babylonian legal contracts almost 4,000 years ago. The skin on the fingers and palms appears as grooves and ridges when observed under a microscope. A unique fingerprint is produced by the patterns of these friction skin ridges. Visible fingerprints can be deposited on solid surfaces. Colored inks have been used to deposit fingermarks on documents. Herein, we show that alkaline phosphatase can be used to transfer prints from fingers or palm to nitrocellulose or polyvinylidene difluoride membranes. The prints can be detected by using the nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate method of detection.

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Figures

Figure 1:
Figure 1:
Thumbprint using alkaline phosphatase and detection with NBT/BCIP
Figure 2:
Figure 2:
Index fingerprint using alkaline phosphatase and NBT/BCIP detection system
See this image and copyright information in PMC

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