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. 1989 Oct 1;263(1):297-9.
doi: 10.1042/bj2630297.

A kinetic investigation of the acyl-CoA oxidase reaction with the use of a novel spectrophotometric assay. Inhibition by acetyl-CoA, CoA and FMN

R Hovik  1 , H Osmundsen
Affiliations

A kinetic investigation of the acyl-CoA oxidase reaction with the use of a novel spectrophotometric assay. Inhibition by acetyl-CoA, CoA and FMN

R Hovik et al. Biochem J. .

Abstract

A direct-reading spectrophotometric assay for acyl-CoA oxidase activity is described. The assay is based on the strong absorption at 300 nm of deca-2-trans,4-cis-dienoyl-CoA, the product of oxidation of dec-4-cis-enoyl-CoA. By use of this assay, acetyl-CoA, CoA and FMN were found to be inhibitors of acyl-CoA oxidase, but with distinctly different kinetic characteristics.

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References

    1. J Biol Chem. 1969 Jul 10;244(13):3514-20 - PubMed
    1. Biochem J. 1989 May 15;260(1):215-20 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2043-6 - PubMed
    1. FEBS Lett. 1979 Mar 15;99(2):292-6 - PubMed
    1. Biochem Biophys Res Commun. 1979 Apr 27;87(4):1200-6 - PubMed

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