Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Mar;75(3):1329-33.
doi: 10.1073/pnas.75.3.1329.

Composition of vacuoles and sarcoplasmic reticulum in fatigued muscle: electron probe analysis

H Gonzalez-Serratos, A V Somlyo, G McClellan, H Shuman, L M Borrero, A P Somlyo

Composition of vacuoles and sarcoplasmic reticulum in fatigued muscle: electron probe analysis

H Gonzalez-Serratos et al. Proc Natl Acad Sci U S A. 1978 Mar.

Abstract

Electron probe analysis, cryo-ultramicrotomy, and freeze-substitution were used to determine the nature of vacuolation and the subcellular composition in fatigued frog skeletal muscle fibers. The vacuoles caused by fatigue were part of the T-tubule system and contained high concentrations of NaCl. The calcium concentration in the terminal cisternae was higher than previously measured normal resting values. Mitochondrial calcium content was relatively low (mean +/- SEM, 2 +/- 2 mmol/kg dry weight). Fiber NaCl was increased. It is concluded that fatigue is not due to the depletion of calcium stores from the terminal cisternae or to uncoupling of mitochondria due to calcium loading but may be caused by multiple mechanisms including failure of the T-tubule action potential.

PubMed Disclaimer

References

    1. Ultramicroscopy. 1976 Sep-Oct;1(4):317-39 - PubMed
    1. J Physiol. 1975 Dec;253(2):321-39 - PubMed
    1. Am J Physiol. 1976 Aug;231(2):430-3 - PubMed
    1. J Physiol. 1976 Dec;263(2):215-38 - PubMed
    1. J Physiol. 1960 Sep;153:370-85 - PubMed

Publication types