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Comment
. 2015 Jun;71(Pt 6):1226-7.
doi: 10.1107/S1399004715008317. Epub 2015 May 23.

Advances in membrane protein crystallography: in situ and in meso data collection

Simone Weyand  1 , Christopher G Tate  2
Affiliations
Comment

Advances in membrane protein crystallography: in situ and in meso data collection

Simone Weyand et al. Acta Crystallogr D Biol Crystallogr. 2015 Jun.

Abstract

Membrane protein structural biology has made tremendous advances over the last decade but there are still many challenges associated with crystallization, data collection and structure determination. Two independent groups, Axford et al. [(2015), Acta Cryst. D71, 1228–1237] and Huang et al. [(2015), Acta Cryst. D71, 1238–1256], have published methods that make a major contribution to addressing these challenges.

Keywords: commentary; in meso crystallization; in meso data collection; in situ crystallization; in situ data collection; membrane proteins.

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Figures

Figure 1
Figure 1
Two solutions for collecting in situ diffraction data from crystallization plates. (a) The set-up at SLS for IMISX (Huang et al., 2015 ▸). (b) The set-up at Diamond for data collection from 96-well plates (Axford et al., 2015 ▸).
See this image and copyright information in PMC

Comment on

References

    1. Axford, D. et al. (2012). Acta Cryst. D68, 592–600. - PMC - PubMed
    1. Axford, D., Foadi, J., Hu, N.-J., Choudhury, H.G., Iwata, S., Beis, K., Evans, G. & Alguel, Y. (2015). Acta Cryst. D71, 10.1107/S139900471500423X. - PMC - PubMed
    1. Bill, R.M., Henderson, P. J., Iwata, S., Kunji, E.R., Michel, H., Neutze, R., Newstead, S., Poolman, B.Tate, C.G. & Vogel, H. (2011). Nat. Biotechnol. 29 335–340. - PubMed
    1. Huang, C.-Y., Olieric, V., Ma, P., Panepucci, E., Diederichs, K., Wang, M. & Caffrey, M. (2015). Acta Cryst. D71, 10.1107/S1399004715005210. - PMC - PubMed

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