Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jun 10:21:1687-92.
doi: 10.12659/MSM.893522.

MiR-506 Over-Expression Inhibits Proliferation and Metastasis of Breast Cancer Cells

Fei Yu  1 , Mingli Lv  1 , Dan Li  1 , Haidong Cai  1 , Lishui Ma  1 , Qiong Luo  1 , Xueyu Yuan  1 , Zhongwei Lv  1
Affiliations

MiR-506 Over-Expression Inhibits Proliferation and Metastasis of Breast Cancer Cells

Fei Yu et al. Med Sci Monit. .

Abstract

Background: This study aimed to investigate the relationship between miR-506 and proliferation and migration of breast cancer cells.

Material and methods: MiR-506 mimics, inhibitor, and negative control (NC) were transfected into MDA-MB-231 breast cancer cells. Cell proliferation, cell counting, colony formation assay, and Transwell assay were applied to evaluate the proliferation and migration of breast cancer cells. Data are shown as mean ± standard deviation and the experiment was performed 3 times. Statistical analyses were performed with SPSS version 10.0.

Results: At 1 day after transfection, cell proliferation detected by CCK-8 assay was significantly promoted in miR-506 inhibitor when compared with the miR-506 mimics group and the NC group (P<0.05). At 3 days or 5 days after transfection, cell proliferation was markedly inhibited in the miR-506 mimics group, and miR-506 inhibitor was still significantly promoted. Cell counting with a hemocytometer showed similar results to cell proliferation. Colony formation assay showed that the number of colonies in the miR-506 mimics group was significantly smaller than that in the miR-506 inhibitor group and NC group. Transwell assay revealed that the number of migrated cells in miR-506 mimics was markedly smaller than that in the miR-506 inhibitor group and NC group.

Conclusions: MiR-506 over-expression significantly inhibits the proliferation, colony formation, and migration of breast cancer cells. miR-506 over-expression may thus be able to improve the malignant phenotype of breast cancer cells.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Detection of cell proliferation by CCK-8 assay in the miR-506 mimics group, miR-506 inhibitor group, and NC group. 506m: miR-506 mimics; 506I: miR-506 inhibitor; NC: negative control.
Figure 2
Figure 2
Detection of cell proliferation by cell counting in the miR-506 mimics group, miR-506 inhibitor group, and NC group. 506m: miR-506 mimics; 506I: miR-506 inhibitor; NC: negative control.
Figure 3
Figure 3
Colony formation assay of cells in the miR-506 mimics group, miR-506 inhibitor group, and NC group. (A) miR-506 inhibitor; (B) miR-506 mimics; (C) NC
Figure 4
Figure 4
Colonies were counted with Image J software. MiR-506m: miR-506 mimics; miR-506I: miR-506 inhibitor; miR-374am: miR-374a mimics; miR-374a I: miR-374a inhibitor; miR-374bm: miR-374b mimics; miR-374b I: miR-374b inhibitor. * P<0.05
Figure 5
Figure 5
Transwell invasion assay of MDA-MB-231 cells in the miR-506 mimics group, miR-506 inhibitor group, and NC group. (A) NC; (B) miR-506 mimics; (C) miR-374a mimics.
Figure 6
Figure 6
Detection of cell density with Image J software. MiR-506m: miR-506 mimics; miR-506I: miR-506 inhibitor. * P<0.05
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Bartel DP. MicroRNAs: target recognition and regulatory functions. Cell. 2009;136:215–33. - PMC - PubMed
    1. Bohnsack MT, Czaplinski K, Gorlich D. Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs. RNA. 2004;10:185–91. - PMC - PubMed
    1. Yu M, Bardia A, Aceto N, et al. Cancer therapy. Ex vivo culture of circulating breast tumor cells for individualized testing of drug susceptibility. Science. 2014;345:216–20. - PMC - PubMed
    1. Aceto N, Bardia A, Miyamoto DT, et al. Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis. Cell. 2014;158:1110–22. - PMC - PubMed
    1. Liang Z, Sun XY, Xu LC, Fu RZ. Abnormal expression of serum soluble E-cadherin is correlated with clinicopathological features and prognosis of breast cancer. Med Sci Monit. 2014;20:2776–82. - PMC - PubMed

Publication types