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. 2015:2015:658519.
doi: 10.1155/2015/658519. Epub 2015 Apr 28.

Identification and characterization of Chlamydia abortus isolates from yaks in Qinghai, China

Zhaocai Li  1 , Xiaoan Cao  1 , Baoquan Fu  1 , Yilin Chao  2 , Jinshan Cai  2 , Jizhang Zhou  1
Affiliations

Identification and characterization of Chlamydia abortus isolates from yaks in Qinghai, China

Zhaocai Li et al. Biomed Res Int. 2015.

Abstract

Recently, the yak population has exhibited reproductive disorders, which are considered to be associated with Chlamydia abortus (C. abortus) in Qinghai, China. In this study, a total of 9 aborted fetuses (each from a different herd) and 126 vaginal swab samples from the 9 herds were collected and analyzed. C. abortus DNA was detected from all of the 9 aborted fetuses and 30 of the 126 vaginal swab samples (23.81%) from yak cows in the selected herds. Four C. abortus strains were isolated from embryonated egg yolk sacs inoculated with foetal organ suspensions. The isolated C. abortus strains were further identified, which showed identical restriction profiles with the C. abortus reference strain using AluI restriction enzyme in the RFLP test. Moreover, the isolated C. abortus strains and C. abortus-positive vaginal swab samples were genotyped by multiple loci variable number tandem repeat analysis and all belonged to the genotype 2 group. These findings suggested that C. abortus played a substantial role in yak abortion in Qinghai, China.

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Figures

Figure 1
Figure 1
Detection of Chlamydia DNA from the 9 aborted yak fetuses from different herds. Marker: 100 bp DNA ladder.
Figure 2
Figure 2
Perinuclear chlamydial inclusions (arrows) in McCoy cells when infected with the isolated strain GN-6. ×400, Giemsa.
Figure 3
Figure 3
Identification of C. abortus isolates by PCR-RFLP analysis. (a) Helicase gene clone 8 PCR products. (b) RFLP analysis by AluI restriction enzyme of clone 8 PCR products. (c) omp2 PCR products. (d) RFLP analysis by AluI of omp2 PCR products. Marker: 100 bp DNA ladder.
See this image and copyright information in PMC

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