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. 2015 Jul;31(7):671-2.
doi: 10.1089/aid.2014.0333. Epub 2015 May 18.

Visualizing Interactions Between HIV-1 Nef and Host Cellular Proteins Using Ground-State Depletion Microscopy

Brennan S Dirk  1 , Bryan Heit  1 , Jimmy D Dikeakos  1
Affiliations

Visualizing Interactions Between HIV-1 Nef and Host Cellular Proteins Using Ground-State Depletion Microscopy

Brennan S Dirk et al. AIDS Res Hum Retroviruses. 2015 Jul.
No abstract available

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Figures

<b>FIG. 1.</b>
FIG. 1.
Visualizing the Nef/MHC-I interaction along Rab5-positive endosomes. Here we imaged a HeLa cell utilizing ground-state depletion microscopy. Cells were cotransfected with plasmids expressing the HIV-1 accessory protein Nef and MHC-I fused to bimolecular fluorescence complementation (BiFc) fluorophores. Cells were subsequently stained for Rab5. The Nef/MHC-I BiFc signal (green) is distributed within the cell, colocalizing with Rab5-positive endosomal structures (red). The scale bar represents 2.5 μm.
See this image and copyright information in PMC

References

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