[The expression of retrogene Nanogp8 is related to the biological characteristics of human SGC-7901 gastric cancer cells]

Abstract

Objectives: To explore the effects of NANOGP8 on proliferation, apoptosis, cell cycle, invasion and migration of human SGC-7901 gastric cancer cells.

Methods: Eukaryotic expression vector pEGFP-N1-NANOGP8 and recombinant plasmid shRNA-NANOGP8 were constructed and confirmed by enzyme digestion and sequencing analysis. Then, vector pEGFP-N1-NANOGP8 and recombinant plasmid shRNA-NANOGP8 were transfected into SGC-7901 cells via liposome. The expression of NANOGP8 mRNA and protein were tested by fluorescence microscopy, reverse transcription PCR and Western blotting, respectively. The proliferation of SGC-7901 cells was detected by CCK-8 assay. The apoptosis and cell cycle were examined by flow cytometry. TranswellTM assay proved the changes in the invasion and migration abilities of SGC-7901 cells.

Results: The recombinant plasmids pEGEP-N1-NANOGP8 and pshRNA-NANOGP8 were constructed successfully and transfected into gastric cancer cells. pEGEP-N1-NANOGP8 transfection group showed high expression of NANOGP8, conversely, pshRNA-NANOGP8 transfection group showed low expression of NANOGP8. High expression of NANOGP8 significantly promoted the proliferation of tumor cells, arrested cells cycle in the S phase, decreased apoptotic cells and increased cell invasion and migration obviously. While the low expression group presented with inhibited cell proliferation, cell-cycle arrest in the G0/G1 phase, promoted cell apoptosis and inhibited migration and invasion.

Conclusion: The transcription and expression of retrogene NANOGP8 has a close relationship with the proliferation, cycle, apoptosis, migration and invasion of SGC-7901 gastric cancer cells.