Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1989 Dec;84(1):35-40.
doi: 10.1007/BF00210667.

Increased detectability of somatic changes in the DNA from human tumours after probing with "synthetic" and "genome-derived" hypervariable multilocus probes

P J Lagoda  1 , G Seitz, J T Epplen, O G Issinger
Affiliations
Comparative Study

Increased detectability of somatic changes in the DNA from human tumours after probing with "synthetic" and "genome-derived" hypervariable multilocus probes

P J Lagoda et al. Hum Genet. 1989 Dec.

Abstract

DNA fingerprinting with two minisatellite (33.15, M13) and two simple repeat probes [(GACA)4, (CAC)5/(GTG)s] was performed to screen for somatic changes in the DNA from various solid human tumours in comparison with constitutional DNA from the same patient. Loss of bands or changes in band intensities were observed. Together the probes 33.15 and (CAC)5/(GTG)5 detected deviating fingerprint patterns in 63% of the colorectal carcinomas investigated. In mammary and stomach carcinomas, only 1/11 and 2/11 tumours, respectively, showed differences with either of the three probes, 33.15, (GACA)4 and (CAC)5/(GTG)5.

PubMed Disclaimer

References

    1. Hum Genet. 1986 Nov;74(3):239-43 - PubMed
    1. Somat Cell Mol Genet. 1988 Nov;14(6):519-25 - PubMed
    1. Am J Hum Genet. 1988 Nov;43(5):638-44 - PubMed
    1. Nature. 1985 Mar 7-13;314(6006):67-73 - PubMed
    1. Cell. 1984 Jul;37(3):705-13 - PubMed

Publication types

LinkOut - more resources