Optimization of phenolics and dietary fibre extraction from date seeds

Abstract

This work was conducted to optimise extraction conditions of phenolics and dietary fibre from date seeds. The effects of solvent to sample ratio, temperature, extraction time, number of extractions and solvent type on phenolic extraction efficiency were studied. At two-stage extraction, each stage 1h duration at 45°C with a solvent to sample ratio of 60:1, is considered optimum. Acetone (50%), and butanone were the most efficient solvents for extraction and purification, increasing the yield and phenolic contents of seed concentrate to 18.10 and 36.26g/100g, respectively. The total dietary fibre of seeds (57.87g/100g) increased after water and acetone extractions to 83.50 and 82.17g/100g, respectively. Nine phenolic acids (free and liberated) were detected in seeds with p-hydroxybenzoic (9.89mg/100g), protocatechuic (8.84mg/100g), and m-coumaric (8.42mg/100g) acids found to be among the highest. After extraction and purification, total phenolic acid content increased significantly from 48.64 to 193.83mg/100g. Protocatechuic, caffeic and ferulic acids were the major phenolic acids found in the concentrates. Based on this study, we believe date seed concentrates could potentially be an inexpensive source of natural dietary fibre and antioxidants and possibly used as a functional food ingredient.

Keywords: Antioxidants; Flavonoids; Phenolic acids; Purification; Solvent.