Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 May 27:6:375.
doi: 10.3389/fpls.2015.00375. eCollection 2015.

Function genomics of abiotic stress tolerance in plants: a CRISPR approach

Mukesh Jain  1
Affiliations

Function genomics of abiotic stress tolerance in plants: a CRISPR approach

Mukesh Jain. Front Plant Sci. .
No abstract available

Keywords: CRISPR-Cas9; abiotic stress; gene function; genome editing; transcription.

PubMed Disclaimer

Figures

Figure 1
Figure 1
An overview of the applications of CRISPR-Cas9 system in functional genomics of abiotic stress tolerance. CRISPR-Cas9 system can be used for genome editing (via introduction of point mutations, insertions or deletions), transcriptional regulation (via CRISPR interference, activation, repression or epigenetic modulation) or forward genetics screens (via generation of loss-of-function, knock-down or activation mutants using sgRNA libraries) for understanding the molecular basis of abiotic stress response, which can lead to generation of abiotic stress-tolerant crop plants.
See this image and copyright information in PMC

References

    1. Belhaj K., Chaparro-Garcia A., Kamoun S., Nekrasov V. (2013). Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods 9:39. 10.1186/1746-4811-9-39 - DOI - PMC - PubMed
    1. Bhaya D., Davison M., Rodolphe B. (2011). CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation. Ann. Rev. Genet. 45, 273–297. 10.1146/annurev-genet-110410-132430 - DOI - PubMed
    1. Brooks C., Nekrasov V., Lippman Z. B., Van Eck J. (2014). Efficient gene editing in tomato in the first generation using the clustered regularly interspaced short palindromic repeats/CRISPR-associated9 system. Plant Physiol. 166, 1292–1297. 10.1104/pp.114.247577 - DOI - PMC - PubMed
    1. Cho S. W., Kim S., Kim Y., Kweon J., Kim H. S., Bae S., et al. . (2014). Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases. Genome Res. 24, 132–141. 10.1101/gr.162339.113 - DOI - PMC - PubMed
    1. Feng Z., Zhang B., Ding W., Liu X., Yang D. L., Wei P., et al. . (2013). Efficient genome editing in plants using a CRISPR/Cas system. Cell Res. 23, 1229–1232. 10.1038/cr.2013.114 - DOI - PMC - PubMed

LinkOut - more resources